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ID:33789278
大小:3.70 MB
页数:49页
时间:2019-03-01
《toll样受体2和4在大鼠心肌缺血2f再灌注损伤模型中的运态表达》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、英文摘要DynamicexpressionofTolMikeReceptor2and4inaratmodelofmyocardialischemiareperfusioninjuryABSTRACTobjective:Myocardialischemic/reperfusioninjuryrMI/RI)referstoasyndromewhichwasafterhavingischemiaforsometime,myocardiumnotonlydidnotreducethedegreeofinjuryafterthebloodflowres
2、tored,butalsoaggravatedcomparedwithischemic.MI/R1wascharacterizedby10calandsystemicinflammatoryresponse.Iftheinflammationexpanded,myocardiumwouldbeinjuredandseriouslyaffectedtheleftventricularfunction.MoreandmorestudieshadshownthatMI/R1wasagroupofchronicinflammatorydiseases
3、.Nowadays,scientistsdiscoveryToll—likereceptors(TLRs)playaimportantroleatinflammationreaction.TherelationshipbetweenTLRsandinflammationbecomesmoreandmoreclosely.ToexploretheroleofTLR2andTLR4inmyocardialischemia/reperfusioninjury(MUm)byobservingthedynamicexpressionchangesatm
4、RNAandprotein1evelsearlyaftermyocardialischemia/reperfusion.Methods:Choose42malewistarratswithsimilarweight,andrandomlvdividedtheminto7groups(reperfusionforlh,2h,4h,6h,12h,24h,and7d),eachgrouphad6rats.AnesthetizedeachratwithPentobarbitalsodium.andthenfixeditonaoperatingboar
5、d.Withthehelpofanimal’Sventilator,openingthechest,supplantingtheheart,ligationofleftanteriordescendingcoronary(LAD),finishingtheischemicmodel.Afterischemicforlh,openthechestagain,sheartheligaturewirewithmicrosurgicalscissor,finishingthereperfusionmodel.Afterreperfusionreach
6、esdesiredtime(1,2,4,6,12,24hoursand7days),anesthetizedtheratagain,fixedontheboard,separatedtheskinandsubcutaneoustissueofneck,exposingthetrachea,andincubated.Thenisolatedtherightcarotidandincubated.Beforecuttingofftheheart,ligationoftheca.rdiovascularagaininsitu,injectedabo
7、ut1~1.5mlphthalocyaninebluesolutionintothecarotidartery,itCallbeseenthatthe4英文摘要heartpartlyturnedblueimmediately.Cuttheheart,andputitincleanicesaline,rinsingclean,andplaceditinrefrigeratorusingacleanPetridishesforabout8min.Takentheheartoutandcutitintoslicesofabout2ram,follo
8、wedbyTTCstaining.Finally,usingascannertoscanslicesandsavethepicturesforanalysisthe
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