rhg-csf对大鼠脑缺血再灌注损伤后mmp-3和mmp-9表达的影响

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1、山西医科大学硕士学位论文1．rhG-CSF治疗脑缺血再灌注损伤大鼠，可改善早期神经功能缺损，促进肢体功能恢复。2．rhG-CSF治疗降低了脑缺血再灌注后MMP-3及MMP-9的表达，并降低了血脑屏障通透性，从而减轻脑水肿，这可能是rhG-CSF的脑保护作用机制之一。关键词重组人粒细胞集落刺激因子；脑缺血再灌注；基质金属蛋白酶-3；基质金属蛋白酶-9；血脑屏障2万方数据山西医科大学硕士学位论文TheeffectofrhG-CSFontheexpressionofMMP-3andMMP-9proteinfollowi

2、ngfocalcerebralischemia-reperfusioninjuryinratsAbstractObjectiveToexploretheneuralprotectionmechanismofG-CSFbyobservingthechangeofexpressionofMMP-3、MMP-9andthepermeabilityofblood-brainbarrierinSDratswithcerebralischemia-reperfusioninjurythattreatedwithrecombi

3、nanthumangranulocytecolony-stimulatingfactor（rhG-CSF）.MethodsThirty-nineSprague-Dawleymaleratswererandomlydividedinto3groups,sham-operatedgroup,middlecerebralarteryocclusion/reperfusion(MCAO/R)groupandrhG-CSFtreatmentgroup,with13ratsineachgroup.Establishingmi

4、ddlecerebralarteryocclusionreperfusionratsmodelbylongalineplugmethod.Dissectinginternalcarotidarteryandexternalcarotidarteryinsham-operatedgroup.TherhG-CSFtreatmentgroupreceivedadoseof50ug/kgofrhG-CSFwhichwasintraperitonealinjectedattheonsetofreperfusionafter

5、2hocclusionand24hafterreperfusion.Whilesham-operatedgroupandMCAO/Rgroupwereinjectedthesamevolumeofsaline.Longa5-pointscalewasadoptedtoscoreneurologicalfunctionat48hafterthereperfusion.TheexpressionofMMP-3andMMP-9weredetectedbyimmunohistochemicalmethod.Thecont

6、entofEvenblueinthebrainweredetectedbymicroplatereader.Transmissionelectronmicroscopewasusedtoobservetheultrastructureofblood-brainbarrier.Result1.TheMCAO/RgroupandrhG-CSF-treatmentgrouphaddifferentdegreesoflimbparalysisaftercerebralischemiareperfusion．Compare

7、dwiththeMCAO/Rgroup，theneurologicalfunctionscoresoftherhG-CSF-treatmentgroupreducedobviouslyandthementalstateimproved，thedifferencewasstatisticallysignificant(P<0．05).2.Comparedwithsham-operatedgroup,theexpressionofMMP-3andMMP-9positivecellsweresignificantlyi

8、ncreasedintheischemicacore(striatumandcortex),greyvaluedecreasedintheMCAO/RgroupandrhG-CSF-treatmentgroup(P<0．05).ComparedwiththeMCAO/Rgroup,theexpressionofMMP-3andMMP-9positivecellsweres