欢迎来到天天文库
浏览记录
ID:33788916
大小:3.04 MB
页数:23页
时间:2019-03-01
《rhg-csf对大鼠脑缺血再灌注损伤后mmp-3和mmp-9表达的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、山西医科大学硕士学位论文1.rhG-CSF治疗脑缺血再灌注损伤大鼠,可改善早期神经功能缺损,促进肢体功能恢复。2.rhG-CSF治疗降低了脑缺血再灌注后MMP-3及MMP-9的表达,并降低了血脑屏障通透性,从而减轻脑水肿,这可能是rhG-CSF的脑保护作用机制之一。关键词重组人粒细胞集落刺激因子;脑缺血再灌注;基质金属蛋白酶-3;基质金属蛋白酶-9;血脑屏障2万方数据山西医科大学硕士学位论文TheeffectofrhG-CSFontheexpressionofMMP-3andMMP-9proteinfollowi
2、ngfocalcerebralischemia-reperfusioninjuryinratsAbstractObjectiveToexploretheneuralprotectionmechanismofG-CSFbyobservingthechangeofexpressionofMMP-3、MMP-9andthepermeabilityofblood-brainbarrierinSDratswithcerebralischemia-reperfusioninjurythattreatedwithrecombi
3、nanthumangranulocytecolony-stimulatingfactor(rhG-CSF).MethodsThirty-nineSprague-Dawleymaleratswererandomlydividedinto3groups,sham-operatedgroup,middlecerebralarteryocclusion/reperfusion(MCAO/R)groupandrhG-CSFtreatmentgroup,with13ratsineachgroup.Establishingmi
4、ddlecerebralarteryocclusionreperfusionratsmodelbylongalineplugmethod.Dissectinginternalcarotidarteryandexternalcarotidarteryinsham-operatedgroup.TherhG-CSFtreatmentgroupreceivedadoseof50ug/kgofrhG-CSFwhichwasintraperitonealinjectedattheonsetofreperfusionafter
5、2hocclusionand24hafterreperfusion.Whilesham-operatedgroupandMCAO/Rgroupwereinjectedthesamevolumeofsaline.Longa5-pointscalewasadoptedtoscoreneurologicalfunctionat48hafterthereperfusion.TheexpressionofMMP-3andMMP-9weredetectedbyimmunohistochemicalmethod.Thecont
6、entofEvenblueinthebrainweredetectedbymicroplatereader.Transmissionelectronmicroscopewasusedtoobservetheultrastructureofblood-brainbarrier.Result1.TheMCAO/RgroupandrhG-CSF-treatmentgrouphaddifferentdegreesoflimbparalysisaftercerebralischemiareperfusion.Compare
7、dwiththeMCAO/Rgroup,theneurologicalfunctionscoresoftherhG-CSF-treatmentgroupreducedobviouslyandthementalstateimproved,thedifferencewasstatisticallysignificant(P<0.05).2.Comparedwithsham-operatedgroup,theexpressionofMMP-3andMMP-9positivecellsweresignificantlyi
8、ncreasedintheischemicacore(striatumandcortex),greyvaluedecreasedintheMCAO/RgroupandrhG-CSF-treatmentgroup(P<0.05).ComparedwiththeMCAO/Rgroup,theexpressionofMMP-3andMMP-9positivecellsweres
此文档下载收益归作者所有