ampk-mtor信号通路对自噬的影响在小鼠局灶性脑缺血损伤中的作用

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1、Abstractbetweenp-AMPKandp-mTORlevelswasinverse.AftertreatedwithCompoundC,theexpressionlevelofp-AMPK/LC3/Beclin-1wassignificantlydecreased,whilethep-mTORlevelincreasedsignificantly（p<0.05）.Thedeficiencyofnisslbodieswasreducedcomparedwiththevehiclegroupeachtimeaftertheoc

2、clusion.Neurologicaldeficits,infractareasandbrainwatercontentwerealsosignificantlyrelievedat24htimepointaftertheocclusion（p<0.05）.Conclusion:1.Autophagywasactivatedincorticaltissueafterischemicbraininjuryinmice.2.AMPK-mTORsignalingpathwayswereactivedandtheexpressionwas

3、antagonisticincorticaltissueafterischemicbraininjuryinmice.3.InhibitingAMPKactivitycaninhibitautophagy.4.InhibitingAMPKactivityhadaprotectiveeffectonneuronalinjuryinducedbycerebralischemiainmice.ActivingmTORsignalingpathwaysandreducingautophagicneuronalinjurymayrelativ

4、eonthemechanisms.Keywords：cerebralischemia；AMPK；mTOR；autophagyV目录目录第1章引言.................................................................................................................1第2章小鼠永久性局灶性脑缺血损伤后AMPK-mTOR-自噬的激活情况...........42.1材料和方法.............................

5、........................................................................42.1.1实验材料...............................................................................................42.1.2实验方法..................................................................................

6、.............72.1.3统计学分析.........................................................................................112.2实验结果.......................................................................................................122.2.1pMCAO小鼠大脑缺血半球皮层AMPK和mTOR水平的时向变化......

7、.................................................................................................................122.2.2pMCAO小鼠大脑缺血半球皮层自噬标志蛋白LC3和Beclin-1水平的变化...........................................................................................................142.

8、2.3免疫组化法显示pMCAO小鼠大脑缺血半球皮层AMPK-mTOR-自噬的激活情况...........................................................................