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ID:33758866
大小:1.89 MB
页数:50页
时间:2019-02-28
《血红素氧合酶-1和抵抗素在非酒精性脂肪性肝病中的作用》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、目录1血红素氧合酶-1和抵抗素在非酒精性脂肪肝的作用„„„„„„„„„„„„„„„„„„„„„„„„11.1中文摘要„„„„„„„„„„„„„„„„„„„„„11.2英文摘要„„„„„„„„„„„„„„„„„„„„„41.3前言„„„„„„„„„„„„„„„„„„„„„„„81.4材料与方法„„„„„„„„„„„„„„„„„„„„91.5结果„„„„„„„„„„„„„„„„„„„„„„„181.6讨论„„„„„„„„„„„„„„„„„„„„„„„281.7结论„„„„„„„„„„„„„„„„„„„„„„„361.8参考文献„„„„„„„„„„„„„„„„„„„„„371.9英汉缩略词对照表„„
2、„„„„„„„„„„„„„„„402致谢„„„„„„„„„„„„„„„„„„„„„„„423血红素氧合酶-1和抵抗素在非酒精性脂肪肝的作用(综述)„„„„„„„„„„„„„„„„„„„„„„„43血红素氧合酶-1和抵抗素在非酒精性脂肪性肝病中的作用摘要目的:随着生活水平的提高,饮食习惯的改变。越来越多的人患有非酒精性脂肪肝。非酒精性脂肪肝病(NASH)占平均人口的10%,在肥胖和糖尿病患者中约50%。非酒精性脂肪肝是一种常见的肝脏疾病,最终可发展包括肝硬化和肝癌。非酒精性脂肪肝(NAFLD)的表现与肝脏代谢综合征、内脏肥胖和胰岛素抵抗、氧化应激和炎症紧密联系,这些因素可能是非酒精性脂肪肝发展的
3、重要因素。本文就着重探讨血红素氧合酶-1(HO-1)和抵抗素在非酒精性脂肪性肝病(Nonalcoholicsteatohepatitis,NASH)中的作用。方法:采用雄性SD大鼠60只,体重(200g±20g).常规饲养一周后随机分为4组:对照组15只(普通饲料喂养)和非酒精性脂肪肝模型组15只,血红素氧合酶-1抑制组15只(通过高脂饮食喂养4周后与每周末进行尾静脉注射锌原卟啉抑制)和抵抗素抑制组15只(通过高脂饮食喂养4周后用西洋参夜灌胃),分别于四周末,八周末,十二周末分别处死各组老鼠5只。对大鼠的腹主动脉取血,测天门冬氨酸转氨酶(AST)、丙氨酸氨基转移酶(ALT)、血脂总胆固醇(TC
4、)、甘油三酯(TG)、肿瘤坏死因子(TNF一α)、高密度脂蛋白胆固醇(HDL—C)、低密度脂蛋白胆固醇(LDL—C);对大鼠肝脏进行HE染色,光镜下进行肝组织病理变化的观察;对大鼠肝脏组织切片行免疫染色后,在光镜下进行血红素氧合酶-1的观察。血红素氧合酶-1的阳性细胞所占百分数和着色深浅可表明血红素氧合酶-1在大鼠肝脏组织表达的差异;用RT—PCR方法检测大鼠肝脏组织抵抗mRNA的表达。结果:非酒精性脂肪肝模型成功后观察各组变化,非酒精性脂肪肝模型组天门冬氨酸转氨酶(AST)、丙氨酸氨基转移酶(ALT)、血脂总胆固醇(TC)、甘油三酯(TG)、肿瘤坏死因子(TNF一α)、低密度脂蛋白胆固醇(L
5、DL—C)随着脂肪肝的严重程度增加。非酒精性脂肪肝模型组肝脏病理脂肪变性,坏死,炎症细胞侵润;血红素氧合酶-1抑制组肝脏病理改变较模型组更严重的脂肪变性,血红素氧合酶-1在肝脏组织的着色颜色较浅,8周末,十二周末肝脏脂肪变性较非酒精性脂肪肝模型组严重;西洋参灌胃组8周末,十二周末肝脏病理脂肪改变较非酒精性脂肪肝模型组减轻,大鼠肝脏组织抵抗素mRNA的表达较非酒精性脂肪肝模型组减少。结论:血红素氧合酶-1和抵抗素在非酒精性脂肪肝大鼠中含量都高于正常组,但其作用却不同。血红素氧合酶-1可延缓或阻止非酒精性脂肪肝的进展,血红氧合酶-1(HO-1)是降解血红素生成一氧化碳,自由铁,胆绿素的关键酶。血红
6、素的产物具有抗炎症、抗氧化、防止肝细胞坏死,抗氧化应激的作用。抵抗素来源于脂肪细胞,抵抗素会通过阻止或降低肝糖原的输入从而发生胰岛素抵抗。抵抗素在肥胖患者含量增加,与内脏脂肪性肥胖更是有密切关系,抵抗素可能是导致脂肪肝发展的因素。抵抗素通过胰岛素抵抗可加重非酒精性脂肪肝的形成,改善胰岛素抵抗可延缓非酒精性脂肪肝的发展。关键词:非酒精性肝病;血红素氧合酶-1;氧化应激;胰岛素抵抗;抵抗素TheeffectofHemeoxygenase-1andresistinonpathogenesisofnonalcoholicfattyliverdiseaseabstractObjective:withth
7、eimprovementoflivingstandards,andthechangeofeatinghabits,moreandmorepeoplearesufferfromnonalcoholicfattyliverdisease.Nonalcoholicsteatohepatitis(NASH)accountsforanaverage10%ofthepopulation,especiallyi
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