内质网应激反应对大鼠肺纤维化形成的影响及部分机制的研究

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1、目录英文缩略词表..............................................1中文摘要..................................................3英文摘要..................................................6正文实验一内质网应激相关蛋白在平阳霉素诱导的大鼠肺纤维化模型中的动态变化前言.....................................................9实验材料.............................

2、....................10实验方法.................................................13结果.....................................................22讨论.....................................................26结论.....................................................28参考文献......................................

3、...........28实验二毒胡萝卜素对人胚肺成纤维细胞凋亡影响的初步探讨前言.....................................................31实验材料.................................................31实验方法.................................................341结果.....................................................40讨论........................

4、.............................45结论.....................................................47参考文献.................................................47附录.....................................................49致谢.....................................................50综述：内质网应激反应在肺纤维化中的研究进展............

5、...512英文缩略词Abbreviation英文缩写英文全称中文全称AAbsorbance吸光度APAmmoniumpersulfate过硫酸铵BpBasepair碱基对BLMA5BleomycinA5平阳霉素cDNAComplementaryDNA反转录DNACHOPCCAAT/enhancerbindingproteinhomologousCCAAT增强子结合蛋protein白同源蛋白DMEMDuibecco’smodifiedEagle’smediumDulbec改良的Eagle培养液DEPCDiethylpyrocarbonate焦碳酸乙二脂DMSODimethy

6、lsulfoxide二甲基亚砜EBEthidiumbromide溴化乙啶ECMExtracellularmatrix细胞外基质ERSEndoplasmicreticulumstress内质网应激FCSFetalcalfserum胎牛血清FBFibroblast肺成纤维细胞GAPDHGlyceraldehyde-3-phosphatedehydrogenase3-磷酸甘油醛脱氢酶GRPGlucoseregulatedprotein葡萄糖调节蛋白1HEHematoxylinEosin苏木精-伊红染色mlMilliliter毫升mgMilligram毫克minMinute分MRC

7、-5Humanembryoniclungfibroblast人胚肺成纤维细胞PAGEPolyacrylamidegelelectrophoresis聚丙烯酰胺凝胶电泳PBSPhosphatebufferedsaline磷酸盐缓冲液PFPulmonaryfibrosis肺纤维化PVDFPolyvinylideneFluoride硝酸纤维素膜PIPropidiumiodide碘化丙啶rpmRevolutionsperminute转/分RT-PCRReversetranscriptionRT-PCR逆转录-聚合酶链式反应