重组人血管内皮抑制素yh-16联合放射治疗对肺腺癌a549移植瘤的抑制作用与机制分析

《重组人血管内皮抑制素yh-16联合放射治疗对肺腺癌a549移植瘤的抑制作用与机制分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、华中科技大学硕士学位论文（12.20±1.11）%、（14.75±0.91）%、（16.48±0.85）%、（20.52±1.89）%、（24.45±1.48）%，（P＜0.01）。放疗+Endostar组bFGFmRNA表达水平和VEGF表达水平明显低于对照组、Endostar组和放疗组。结论1.Endostar明显提高了放射治疗对肺腺癌A549移植瘤的疗效，其机制与Endostar通过下调bFGF转录水平和VEGF表达水平抑制肿瘤新生血管的形成、协同-1放射治疗促进肿瘤细胞凋亡相关。2.DDP（3mg·kg

2、，d3）联合放射治疗治疗肺腺癌-1-1A549移植瘤和Endostar(10mg·kg·d，d1-14)联合放射治疗疗效相当。关键词肺肿瘤;重组人血管内皮抑制素;放射治疗;移植瘤;人肺腺癌细胞系A549;碱性成纤维细胞生长因子;血管内皮生长因子4华中科技大学硕士学位论文TheInhibitionofRh-endostatin（YH-16）inCombinationwithRadiotherapyonLungAdenocarcinomaA549inMiceanditsMechanismsGraduateWuHui

3、-TaSupervisorProf.ChenYuanTongjiHospitalCancerCenter,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430030ABSTRACTObjectiveThemajorgoalofthisstudywastoevaluatetheinhibitoryeffectofEndostarincombinationwithradiotherapyonlungadenocarcinomaA

4、549inBALB/cnudemice.Meanwhile,theratesoftumorcellapoptosisandthelevelsofbFGFmRNAandVEGFexpressionweredetectedtoresearchtheinteractionmechanismsofcombinedtherapy.Inaddition,acomparisonbetweentheeffectofEndostarplusradiotherapyandcisplatinplusradiotherapyonA54

5、9lungadenocarcinomamodelswascarriedout.MethodsThetransplantationtumormodelsofA549lungadenocarcinomawereestablished.Allmicewererandomizedinto5groups（n=6）whenthelargestdiameterof-1tumorreachesat1.0cm.Endostargroup:miceweregiven10mg·kgofEndostardailybyperitonea

6、linjectionfor14days.Controlgroup:miceweregivenbyequalvolumesofnormalsodiumfor14day.Radiotherapygroup:miceweretreatedby10Gyelectronbeamirradiationatthethirdday.Endostarplusradiotherapygroup:miceweregivendailyby-1peritonealinjectionwith10mg·kgofEndostarfor14da

7、y,inaddition,theyweregiven10Gyelectronbeamirradiationatthethirdday.DDPplusradiotherapygroup:twohoursbeforemiceweregiven10Gyelectronbeamirradiationatthethirdday,theyweregiven-13mg·kgcisplatinbyperitonealinjection.Wemeasuredthelargestdiameterandthevertical5华中科

8、技大学硕士学位论文thdiameteroftumoratdifferenttimepoints.Atthe16day,allmicewereexecuted.Thetumorswereappliedtoanalysisofratesoftumorcellapoptosis,andtheexpressionlevelsofbFGFmRNAbyRT-PCRandVEGFbyIHCwerea