重组人内皮抑素对肺鳞癌细胞抑制作用的体外

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1、重组人内皮抑素对肺鳞癌细胞抑制作用的体外】目的以k562/dox和mcf7/dox细胞为对象，探讨异汉防己碱对化疗药物阿霉素（dox）的增敏作用及其作用机制。方法采用mtt法检测异汉防己碱的内在细胞毒性及其对阿霉素的增敏作用，并以rf值评价其增敏效果。应用流式细胞术（fcm）检测细胞膜上pgp的表达以及细胞内dox和罗丹明123（rh123）的蓄积量。结果异汉防己碱在10μg/ml的无毒剂量可明显增强dox的细胞毒性。k562/dox和mcf7/dox细胞膜上pgp均呈强阳性表达，但异汉防己碱对该p

2、gp表达水平无明显影响。异汉防己碱可使k562/dox和mcf7/dox细胞内dox和rh123的荧光密度（fi）均明显增加，由此证明异汉防己碱可有效抑制pgp的功能。结论异汉防己碱可通过抑制pgp的功能而增强阿霉素的敏感性，从而有效逆转肿瘤细胞的多药耐药性（mdr），它可能成为有效多药耐药逆转剂的候选药物。【关键词】异汉防己碱；阿霉素；肿瘤细胞；多药耐药性abstract：objectivetoexploretheeffectandmechanismofisotetrandrinetoenhanc

3、edoxorubicin(dox)sensitivityofk562/doxandmcf7/doxcells.methodstheactivityofisotetrandrinetoenhancedoxorubicincytotoxicitytt[3(4,5dimethylthiazol)2,5diphenyltetrazoliumbromide]assayandevaluatedbythereversalfold(rf)values.thelevelofpglycoprotein(pgp)e

4、xpressionandintracellularaccumulationofdoxorubicinandrhodamine123(rh123)etry(fcm).resultsthedoxorubicininducedcytotoxicityl.pgpcf7/doxcells,butthelevelofpgpexpressionulationofdoxandrh123ormultidrugresistance(mdr)byinhibitingthefunctionofpgpinvitro,sug

5、gestingthatitmaybeeacandidateofeffectivemdrreversingagentsincancerchemotherapy.keyorcells;multidrugresistanceintroductioncancermultidrugresistance(mdr)isoneofthemajorobstaclesforthesuccessofchemotherapy.itisrelatedtoa170kdaplasmamembraneprotein,pglycoprot

6、ein(pgp)[1].pgpfunctionsasanatpdependentdrugstransporterotherapydrugsinconjunctioneoftumortreatmentdr.accordingly,avarietyofdrugshavebeenreportedasagentsforoveringmdr.hoanydrugsmakethemincapableeffectivelyappliedintheclinic.therefore,developmentofsafean

7、deffectivemdrreversingagentsiseagerlyrequired.isotetrandrine,abisbenzylisoquinolinealkaloidextractedfromtraditionalchinesedrugmahonia,isdiastereoisomeroftetrandrine(tet)[2].previousreportshaveshoormdr[35].inthisstudy,drink562/doxandmcf7/doxcells.1materia

8、lsandmethods1.1drugsandreagentsisotetrandrinejintairongmedicineco.ltd.,guangdong,china;3(4,5dimethylthiazol)2,5diphenyltetrazoliumbromide(mtt)andrhodamine123(rh123)sigmaco.,usa;antihumanpgpmousemabandf