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ID:33732566
大小:1.04 MB
页数:41页
时间:2019-02-28
《老龄大鼠心脏缺血预处理后hsp27与αb-crystallin的变化》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、军医进修学院硬士学位论文TheChangeofHSP27andaB-CrystallininAgedRatHeartAfterIschemicPreconditioningAbstractobjective:Themyocardialischemla-reperfusioninjury(鳓isamaininterferen啪inthereperfilsiontherapyforacutemyocardialinfarctionpatients.andischemicpreconditioning(mC)canobviouslyreduce
2、theinjuryofeardiacmuscle.Theprotectionofsmallheatshockprotefinstomyocardiumfromischemicpreconditioninginadultratshasalreadybeenknown,butsomestudiesshowedthatthisprotectionhadnotbeenfoundinagedrats.Previousstudieshadshownthatthesmallheatshockproteins(sHSPs)protectthemyocar
3、diumfromI/R-induceddamage.TolearntherelationshipbetweentheexpressionofsHSPsandagingofratsduringIPC,thepresentstudywasdesignedtoinvestigatetheexpressionofalpha-Bcrystallin(aBC)andHSP27inthemyocardiumduring碑CbothinmRNAlevelandproteinlevelinad
4、lltandyoungratstoexplaintheesse
5、lloeandregulationofdescendingadaptabilityandascendingsensitivitytoinjuryinagedheart,Materialndmethods:1)Adultmale(8-20weekold)andagedmale(20-24monthsold)SDratswererandomlydividedintotwogroups:controlledgroup(Ctrl)andischcmicprecnndi-tionedgroup(口C).IntheCtrlgroup,rats’bre
6、astwfffeopenedwi也continuousperfⅥsingfor60minutes,whileintheIPCgroup,ratsweretreatedwithischemia(5minuteseach)3times,托pt时.usedfor5minutes.After3timesofischemiaandreperfualonanteriorwallofleftventricletnmtedwithischemiaandposteriorwallofleftventricletreatedwilhnon-ischemiaw
7、eretakenin0mln,5rain,15min.45rainand60min,andtherewere4ratsineachgroup.ThenthesesamplesWereputintoliquidnitrogenfor5minuteandfi,ozeninto一70"(2refrigerator.2)ThedistributionofHSP27anduBCproteiusinsolubleendochylemaand4军医进修学院硬士学位论文insolubleorganelle:thehearttissueswe糟placed
8、intosplitbuffers,andthenthesolubleproteinandinsolubleproteinwerecollectedanddetectedviawesternblot.3)ThelocationofHSP27andctBCproteinsinheartcells:thefreshdefrostedhearttissuesWel*t。markedwithHSP27ando.BCproteinsviailnm明onuo“怒c吼I∞methods,andthenobservedunderlasereonfoea/m
9、icroscopy.4)mRNAexpressionofHSP27andaBCproteinsindifferentlimewithisehemiatreatment:themRNAexpre
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