老龄大鼠心脏缺血预处理后hsp27与αb-crystallin的变化

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1、军医进修学院硬士学位论文TheChangeofHSP27andaB-CrystallininAgedRatHeartAfterIschemicPreconditioningAbstractobjective：Themyocardialischemla-reperfusioninjury(鳓isamaininterferen啪inthereperfilsiontherapyforacutemyocardialinfarctionpatients．andischemicpreconditioning(mC)canobviouslyreduce

2、theinjuryofeardiacmuscle．Theprotectionofsmallheatshockprotefinstomyocardiumfromischemicpreconditioninginadultratshasalreadybeenknown，butsomestudiesshowedthatthisprotectionhadnotbeenfoundinagedrats．Previousstudieshadshownthatthesmallheatshockproteins(sHSPs)protectthemyocar

3、diumfromI／R-induceddamage．TolearntherelationshipbetweentheexpressionofsHSPsandagingofratsduringIPC，thepresentstudywasdesignedtoinvestigatetheexpressionofalpha-Bcrystallin(aBC)andHSP27inthemyocardiumduring碑CbothinmRNAlevelandproteinlevelinad

4、lltandyoungratstoexplaintheesse

5、lloeandregulationofdescendingadaptabilityandascendingsensitivitytoinjuryinagedheart，Materialndmethods：1)Adultmale(8-20weekold)andagedmale(20-24monthsold)SDratswererandomlydividedintotwogroups：controlledgroup(Ctrl)andischcmicprecnndi-tionedgroup(口C)．IntheCtrlgroup,rats’bre

6、astwfffeopenedwi也continuousperfⅥsingfor60minutes，whileintheIPCgroup,ratsweretreatedwithischemia(5minuteseach)3times，托pt时．usedfor5minutes．After3timesofischemiaandreperfualonanteriorwallofleftventricletnmtedwithischemiaandposteriorwallofleftventricletreatedwilhnon-ischemiaw

7、eretakenin0mln,5rain,15min．45rainand60min，andtherewere4ratsineachgroup．ThenthesesamplesWereputintoliquidnitrogenfor5minuteandfi,ozeninto一70"(2refrigerator．2)ThedistributionofHSP27anduBCproteiusinsolubleendochylemaand4军医进修学院硬士学位论文insolubleorganelle：thehearttissueswe糟placed

8、intosplitbuffers,andthenthesolubleproteinandinsolubleproteinwerecollectedanddetectedviawesternblot．3)ThelocationofHSP27andctBCproteinsinheartcells：thefreshdefrostedhearttissuesWel*t。markedwithHSP27ando．BCproteinsviailnm明onuo“怒c吼I∞methods,andthenobservedunderlasereonfoea／m

9、icroscopy．4)mRNAexpressionofHSP27andaBCproteinsindifferentlimewithisehemiatreatment：themRNAexpre