双酚a诱导成年sd大鼠睾丸生殖细胞凋亡与fas%2ffasl和c-myc基因表达的关系

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1、英文摘要virusinducedinvitroculturedalonetesticularspermcells，bisphenolA(BPA)inducedtesticulargermcellapoptosisandFas／FasLrelationswithC—mycgene．Impactonmalefertilityinrecentyearsresearchhashighlybeenglobalscientistsattentionandresearch．ThisexperimentusingbisphenolAlavageinfectedmaleSDrats

2、modeltomonitorthegermcellapoptosisandFas／FasLwithC—mycgeneexpressionintestisofrats，andtofurtherexploretheFas／FasLandC-mycgenemediatedcorrelationbetweengermcellapoptosispathway．ForBPAfundamentalcausesofthegermcellapoptosismechanismprovidesthetheoreticalbasis，andforthepreventionandcontr

3、olforchemicalpollutioncausedbyanimalandhumanreproductiveabilitytoprovidenewcluestoexpectfurtherinordertopreventandresolveenvironmentalhormonebisphenolA(BPA)provideAbasisforhumanmalefertility．Materialsandmethods：1experimentalanimalsandmaterials：experimentalanimalstochoosecleanlevel8wee

4、ksadultmaleSD(Spraguewrote—Dawley)rats，weightin180-200一g．Dividealltheratscageintheanimalroomforaweekaftertheexperiment，thesimulatednaturallightingfor12hoursaday，arefreetogatherthefoodfullpricefeedparticles，drinkingwater，everycanisterregularly，canisterto5timesaweek，andmightbeusedinexpe

5、rimentsafter8weeks．2groupingandinfectedmethod：experimentalanimalswillonly40ratswererandomlydividedintofourgroups：controlgroup，lowdosegroup，middledosegroupandhighdosegroup，10ineachgroup．EachgroupseparatelyincomoilmixtureconcentrationofdifferentdosesofBPAlavageinfected，lowdosegroupwasgi

6、ven2mg／kgBPAlavage，mediumdosegroupwasgiven20mg／kgBPAlavage，highdosegroupwasgiven200mg／kgBPAlavage，controlthedailyirrigationamountofcornoil．3experimentalspecimenprocessing：aftertheinfectedratsweighing，andthenexecutedtoweighingtheepididymistesticularvisceracoefficientcalculation，testicu

7、larneutralformaldehydefixation，embedding，sectioninglinesofHEstainingandimmunohistochemicalstaining，thecontrastanalysis5英文捂要ofinfectedgroupandthecontrolgroup，theapplicationofSPSSsoftwareforanalysis．Results：1Theratbehaviorchange：wefoundthatthehi曲dosegroupofratsshowed：behairmessy，decreas

8、edact