双酚a诱导小鼠睾丸生殖细胞凋亡的分子机制

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1、pathwayplayedakeyroleinBPA-mediatedgermcellapoptosisintestes,wedeterminedtherelativeproteinexpressionintheFas/FasLapoptoticpathwaybyimmunoblotting.Thepresentresultsindicatedthatadose-dependentincreaseinthelevelofFasandFasLwasobservedintestesofmiceexposedtoBPA

2、(160and480mg/kg)duringpuberty.Inaddition,pubertalBPAexposureevokedtheactivationofcaspase-8andcaspase-3intestes.ToinvestigatewhetherthemitochondrialsignalingpathwaywereinvolvedinBPA-mediatedgermcellapoptosisintestes,weanalyzedtherelativeproteinexpressioninthem

3、itochondrialsignalingpathwaybyimmunoblotting.ThecurrentresultsshowedthatpubertalBPAexposurecausedthetranslocationofcytochromec(Cytc)frommitochondriaintocytosol.Inaddition,pubertalBPAexposuremarkedlyupregulatedthelevelofBaxandactivecaspase-9inmousetestes.Furth

4、erstudyindicatedthatpubertalBPAexposuresignificantlyupregulatedtheexpressionofBaxintestesinadose-dependentmanner.Importantly,thelevelofBaxinmitochondrialfractionsoftesticularlysateswassignificantlyincreasedinmiceexposedtoBPAduringpuberty.Unexpectedly,pubertal

5、BPAexposuredidnotreduceproteinleveloftheantiapoptoticmemberBcl-2intestes.Actually,theexpressionofBcl-2wasslightlyupregulatedintestesofmiceexposedtoahighdoseofBPAduringpuberty.However,theratiooftheproapoptoticmemberBaxvstheantiapoptoticmemberBcl-2wassignifican

6、tlyincreasedintestesofmiceexposedtoBPAduringpuberty.ConclusionPubertalBPAexposureinducedgermcellapoptosisintestesofmalemice.ApoptoticgermcellswereobservedmainlyinstagesVII–VIIIseminiferoustubulesintestes.Inaddition,BPA-mediatedgermcellapoptosisintestesdepends

7、onnotonlytheFas/FasLsignalingbutalsothemitochondrialapoptoticpathway.KeywordsbisphenolA/testis/cellapoptosis/mouse/Fas/FasLreceptorpathway/mitochondrialsignalingpathway5双酚A诱导小鼠睾丸生殖细胞凋亡的分子机制1.前言男性不育是人口与生殖健康领域中急需解决的重大科学问题。WHO不育症防[1]治专题组的研究结果表明，精液质量低下是男性不育的最重要原因

8、之一。1992年，丹麦学者Carlsen调查发现，近50年男性精子数量和精液量分别下降50%，男性[2]隐睾和尿道下裂等生殖道异常发生率亦增加一倍。随后，我国学者张树成等对1981～1996年中国有生育力的成年男性精液质量相关文献分析亦显示，中国男性66精液质量呈明显下降，即精子数由103.2×10个/ml（1983年）降至83.8×10个/ml[3]（1996年），精子