mptp致慢性帕金森病小鼠模型的蛋白质组学研究

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1、重庆医科大学硕士研究生学位论文(IPG—IEF)，andthenverticalflatsodiumdodecylsulphatepolyacrylamidegelelectrophoresis(SDS．PAGE)．⑤Theproteinspotsingelswerevisualizedbysilverstainingprotoc01．⑥Scannedgelsandanalyzedtheimages．Searchedforthedifferentiallydisplayedproteinspotsbycomparingtwogrou

2、psofgelimages．QldentifiedsomeofthedifferentiallydisplayedproteinspotswithMS(massspectrometry)followeddigestedwithenzyme．Results：①Behaviorobservationresults：comparedwiththecontrolgroup，thepoleclimbingtimeofthetestgroupwasobviouslylasting(p<0。05)．ThegrouptreatedwithMPTPd

3、isplayedmarkedlyhypoactivebehaviorandthecontrolgroupdidn’tdisplayparkinsonismbehavior．②Throughthecomparisonofthesubstantianigra2-DEmappings(pH3一l0andpH4-7)ofmousebetweenthePDandcontrolgroups，theresolutionof2-DEmappingswithpH4—7IPGDryStripwasbetterthanthosewithpH3．10IPG

4、DryStrip．③Comparingtwoproteinextractionmethodsby2-DEmappings，wefoundthegroupwithnotanytreatmentwasbetter．@ThroughtheanalysisofproteinspotsgroupwithPDQuest8．0analysissoftware，therewere812+21proteinspotsonthesubstantianigra2-DEimagesofPDmouseintherangeofpH4-7，and775+38on

5、thecontrols．18proteinspotswerehyp-expressionand13proteinspotswerehomo—expressiononthesubstantianigra2-DEmappingsofPDbycomparingthemappingsoftWOgroups，twoproteinspotswere7重庆医科大学硕：L研究生学位论文new．Therewere912士21proteinspotsonthestriatum2-DEimagesofPDpatientsintherangeofpH3—1

6、0，and875士38onthecontrols．6proteinspotswerehyp-expressionand4proteinspotswerehomo—expressiononthestriatum2-DEmappingsofPDbycomparingthemappingsoftwogroups，twoproteinspotswerenew．⑤ThroughtheMSidentifition，thehomo·expressionproteinsspotinsubstantianigrawere0【一enolase．Then

7、ewproteinspotsweretumornecrosisfactorligandsuperfamilymember4andcyclin—dependentkinaseinhibitorlB．Thehomo—expressionproteinspotsinstriatumweremitochondrialfissionregulator1andProteinSI00．A10．ThenewproteinspotswereUbiquitin-likeprotein3precursorandLin．7homologB．Conclusi

8、on：MPTPchronicparkinsonismmodelwasabletorepresentnaturalCouseofParkinson’Sdisease．Theidealsubstantianigra2-DEmappings