co_2c2_气腹对炎症环境下巨噬细胞功能影响的实验分析

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1、Attheearlystageofinflammation,macrophagesundergoactivationandreleaseinflammatoryfactorsandattackedandnon-selectivecytotoxicmoleculars，suchasfreeradicals．Freeradicalsparticipateintheinitialandpropagateofavarietyofdiseases．Whenalargeamountoffreeradicalsformincells，thesefreeradicalmolecules

2、attackthecellmembranesandcauselipidperoxidationandfattyacidsbroken，whichleadtodamagestothestructureofcellmembranesandchangesofmembranepermeability,resultingincelldamage．Therefore，eliminatingandinhibitingfreeradicals，aswellasimprovingtheresistanceofoxidation，areimportantwaysforpreventiona

3、ndtreatmentsofdisease．ToinvestigatethemechanismoftheeffectofC02pneumoperitoneumoninflammato巧factors，westudiedthereleaseofsuperoxideanionandchangesofmitochondrialfunctioninmacrophages／nvitroundertheconditionsofC02pneurnoperitoneum．Accordingtotheresults，weevaluatedthefeasibilityofC02pneumo

4、peritoneuminlaparoscopicsurgeryintheconditionofinflammation．MaterialsandmethodsUsingtheratalveolarmaerophagecelllineNR8383andQueueMulti-gasincubator(UnitedStates)，weprovided99％C02tointerfere、)Iritllthegrowthofmacrophages，whichsimulatedC02pneumoperitoneumenvironment．Pretreatmentofadjustab

5、lemulti—gasincubatorforhypoxicenvironmentbeforetheexperiment：thehypoxicgasmixture(99％C02，1％02)Wasinflatedthroughthestomataattherateof20ml／minandthenthestomatawereclosedafter30min．Pressure．temperatureandrelativehumiditywerecontrolledat15mmHg,21oC．and2％respectively．Test1：TheeffectofC02pneu

6、moperitoneumonsuperoxideanioninratalveolarmacrophageinconditionofinflammation．Chemiluminescencedeterminationoffreeradicalsisoneofthemostsensitivemethods．Theprincipleisthattheelectronoftheproduct,whichisd甜vedinthereactionsbetweenchemiluminescencereagentsandoxygenfreeradicalsandisinexcited

7、state，returntothegroundstatebythenon-radiativetransitionsandemitphotons．WedetectedthereleaseoffreeradicalsinmacrophagesbyuniversalmicroplatespectrophotometerintheconditionofsimulatedC02．5．pneumoperitoneumenvironmentandtheresumednormaloxygenenvironment．ToexplorewhetherC02h