甲硫氨酸酶对胃癌细胞增殖影响

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1、摘要目的：探讨甲硫氨酸酶对胃癌细胞AGS增殖的影响，并探讨甲硫氨酸酶与奥沙利铂联合使用是否会发生协同作用方法：1．利用MTT技术测定被甲硫氨酸酶或者甲硫氨酸联合奥沙利铂处理的胃癌细胞增殖情况。2．利用流式细胞仪测定被甲硫氨酸酶或者甲硫氨酸联合奥沙利铂对胃癌细胞细胞周期的影响。结果：本研究发现甲硫氨酸酶可以抑制胃癌细胞AGS增殖，并促进后者凋亡。甲硫氨酸酶抑制胃癌细胞增殖呈现时间、剂量依从性，培养基中加入甲硫氨酸酶时胃癌细胞凋亡率显著升高(50．280／社6．84％vs12．950／社8．93％，P<0．05)，并且当甲硫氨酸的浓度为

2、0．6Unit／m1培养72h时抑制作用最强烈。本实验还发现在抑制胃癌细胞增殖中奥沙利铂对甲硫氨酸酶起协同作用。培养基中加入甲硫氨酸酶+奥沙利铂比培养基中单纯加入甲硫氨酸酶引起的细胞凋亡更显著(73．730／社8．72％Vs49．260／社8．57％，P

3、effectofL—methioninaseonproliferationofgastrccancercelllineAGS．AndinvestigatethatistherethesynergisticeffectbetweenOxaliplatinandL-methionibase．Methods：t．1．AftertreatedbyL-methiomnaseorL—methioninaseandOxaliplafin，gastriccancercellweresurveyedbyMTTassaytodeterminecellp

4、roliferation．2．AftertreatedbyL·methioninaseorL-methioninaseandOxaliplatin．Weuseflowc”ometrytoobservethedistributioncelllinewhengastriccancercellweretreatedbyL-methioninaseorL-methioninaseandOxaliplatin．Results：OurstudyfoundthatL·methioninasecouldinhibittheproliferation

5、ofgastriccancercelllineAGS，andtheninducedapoptosis．L-methioninaseleadcellgrowthinhibitionandapoptosisinatimeanddosedependentmanner．Thegreasteffectappeared72hafterL-methiomnase(0．6Unit／m1)wasadministered．Andtheratioofapoptosisofexperimentalgroupwassignificantlyhighertha

6、nthecontrolgroup(50．280／社6．84％vs12．950／硅8．93％．P

7、foundthatL—methionibasecouldinhibittheproliferationofgastriccancercelllineAGS．GastriccancercelllineAGSismetlliomne-dependentcell．2．ThesynergisticeffectwerefoundwhencombineL-methionibaseandOxaliplatinKeywords：L—methionibase；Oxaliplatin；Gastrictumor；apoptosisIII《琶爹z目录第1章

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