不同途径移植骨髓间充质干细胞促进创伤性脑损伤大鼠功能恢复和神经再生的研究

《不同途径移植骨髓间充质干细胞促进创伤性脑损伤大鼠功能恢复和神经再生的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、英文摘要performedatl，2，3and4weeksafterTBI．Theotherratsofeachgroupweresacrifcedat2and4weeksafterTBI，ThemorphologychangesofbraintissueswereinvestigatedbyHEstaining；WesternBlotandimmunochemistrywereusedtoevaluatetheexpressionlevelandregionaldistributionofNeⅣegrowthfactor(NGF)andBrain—derive

2、dneurotrophicfactor(BDNF)inthebraintissues；Tostudytheregionaldistributionandthedifferentiationofnewlygeneratingcellsintoneuronsandastrocytes，Immunohistochemicaldouble·-stainingwasusedforSry,MAP--2andGFAP．4QuantitativeassayandStatisticalmethod：Theimmunohistochemicalstainedsectionswere

3、analyzedwiththeaveragepositivecellsdetectedbyImage-ProPlus6．0digitalmedicalimageanalysissystem．ThefilmsofwesternblotwereanalyzedwithODvaluesusingImageJimageanalysissoftware．Forallstatisticalcalculations，weusedSPSS13．0forwindowsandone—wayANOVA．ThereWasstatisticallysignificantiflateral

4、Pvalue

5、ologybecamemoreandmoreuniform，likespindle，thecellswerearrangedparalleltothegrowthorswirlinggrowth．ThirdgenerationofBMSCswereused．ThroughcellgrowthcharacteristicsandmorphologicalidentificatedBMSCs．2PathologicalchangesHEstainingshowedanormalcorticalbraininshamgroup，bloodvessellumenwasn

6、ormal，bloodvesselwallWassmooth，nervecellswerecompleteform；InTBIgroup，itcouldbeseendifferentdegreesofsubarachnoidhemorrhage，extensiverangeofedemainthecerebralcortex，lumenexpansion，capillarycongestion，nervecellswereswelling，messydistributed，andeeven6英文摘要cellssizeshrunkenanddeepkaryopyk

7、nosisdyed；comparewithTBIgroup，thepathologicalchangeswerebothslightlyredusedintransplantgroups．3TheresultsofmNSSandRotarodtestTheresultsofmNSSandRotarodtestinshamgroupwerenormal；comparedwithshamgroup，inallthesametimepointoftheothermodelinggroups，thepointofmNSSwereincreasedandthetimeof

8、Rotarodtestw