硅油对兔眼角膜结构及功能的影响

《硅油对兔眼角膜结构及功能的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、徐州医学院硕士学位论文硅油对免眼角膜结构及功能的影响姓名：樊伟英申请学位级别：硕士专业：眼科学指导教师：牟莉；李明新2012-04徐州医学院硕士学位论文硅油对兔眼角膜结构及功能的影响中文摘要目的探讨硅油对兔眼角膜结构及功能的影响。方法将24只健康成年新西兰大白兔随机分成2组：前房硅油注入组(SIG)20只，空白对照组(CG)4只，均行前房穿刺，注入硅油(SIG)或BSS(CG)O．10ml，手术前后定期行裂隙灯、角膜厚度、角膜内皮细胞摄像，将大白兔处死后行角膜内皮N矿．K+．ArP酶活性检测、台盼蓝．茜素红染色、病理组织学检

2、查、透射电镜及扫描电镜检查。结果角膜内皮细胞密度检查结果中，SIG8周时角膜内皮细胞形态不规则，角膜内皮细胞数目下降，与相应时限CG比较有统计学意义(P

3、细胞无显著改变。结论兔眼前房内硅油停留8周以上可引起角膜内皮细胞发生不可逆的损伤，并且时间越长，损伤越严重。关键词硅油类；内皮，角膜；病理组织学；兔徐州医学院硕士学位论文⋯⋯●●ll·1●■J●』■EIIeCt0ISlnC0ne0nC0rneaIStrUCtUreandIUnCtlon0lrabbiteyesAbstractobjectiveToinVestigate恤e毹ctofsiliconeoilonthecomealstnJcn鹏andf．unction．MethodsTwentyfourhealmywhiterab

4、bits、Ⅳere砌doIIllydiVidedinto咖groups：siliconeoilin恤肌teriorcharllbergroups(SIG)肌dcon仃ol铲oup(CG)．AllrabbitsuIlde刑ent觚teriorch锄beri巧ectionofO．1OmlsiliconcoilorBSS．Slit-l锄pbiomicroscopy，comealnlicknessinstnlmemandComemendotheli啪photographywereperfomledmerthcrabbitswerep

5、erf．omledpre—irljectionarldpost-iIljection．Na十一K+-ATPaseactiVitiesdetection，1时panBlue-AlizariIlredstailling，Histopamologicalexamination，transmissionelec们nmicrosc叩y(TEM)andsca肌ingelectronmicroscopy(SEM)wereperfomedafbermerabbitsweresacrificed．ResultsSince8weekspoSto

6、peratiVely，theendomelialcellsweremarkedlyirregularinsizeaIldsh印e，a11dthenumberofendotllelialcellswaSm缸kcdlydecreased．Thedensityofmecomealendotheli啪inSIGhadstatisticallysignific肌tcomparedwithcontrolgroup(P

7、ksto16weeks(P<0。05)；TheerldotheliaLlcellswercsho、Ⅳedscattcredpa=cchyd锄agesince8、veekspostoperaliVely，which、Verefo眦diIlTrypaIlBlue-舢iz撕nredstaining；Multilayeredre缸．ocomealmembranesgrewgraduallyf如m8weeksaRersu唱ery．Nucleardegeneration，edemaofendoplasmicreticulumalldpa

8、rtofmeendomelialcelldegene俐ona11dlosswerefoulldinTEM；Thereweremoreproductionsofdifrerentlengt}lsatt11ecellssurfaceininju巧areaaIldt11edefo肌edcells