透明质酸复合bmp-2转染骨髓基质干细胞修复骨缺损

《透明质酸复合bmp-2转染骨髓基质干细胞修复骨缺损》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、占塑蔓三垦型奎兰!!!!堑里主盟茎竺兰堡丝苎HyaluronicacidmixedwithBMP一2genetransfectedbonemarrowderivedmesenchymalstemcellsforrepairofdiaphysealdefectsinrabbits彳丑譬删CFBackgroudRepairofthebonedefectswasthegreatchallengefortheclinicalpraetise．Currently，repairofthebonedefect

2、smaybepossiblethankstodevelopmentoftissueengineering．Theconstructionoftissue—engineeredboneshouldrequirethreeelementS：theosteoprogenitorcelis．osteoinductivefactorsandosteoconductivematerials．Thecombinationoftissueengineeringandgeneengineeringcanmeett

3、herequireme几ts，whichwasreferredtoastheidealmethodforrepairofthebonedefectS．ObjectivesToeva]uateosteogenetiCandchondrogeneticeffectivenessofhyaluronicacid(HA)mixedwithadenovirusmediatedhumanbonemorphogeneticprotein一2gene(Adv—hBMP一2)transfectedbonemarr

4、owderivedmesenchymalstemcellS(BMSCs)ofrabbitinvitroandinthemuseleofnudemiceandtoexaminetheeffectivenesSofthiSmethodintherepairofradialshaftdefectsofrabbits．Methods(i)Testinvitro：TheBMSCswerecol1ectedfrombonemarrowofrabbit，culturedinvitro．Therewerefou

5、rgroupsinthetest：I．Adv—hBMP一2transfectedBMSCs+HA．II．Adv—hBMP一2transfectedBMSCs，ⅡI．untransfectedBMSCs+HA，Ⅳ．untransfectedBMSCs．TheosteogenesisoftheseBMSCswereevaluatedbyImmunoprecipitation(IP)／WesternblottingforhBMP一2，RT—PCRforbonesialoprotein(BSP)。ALP

6、activityassayandvonKossastaining．(2)Theosteoinductivetestinthemuscleofnudemice：12nodemice(24sides)weredividedintofourgroups：I．Adv—hBMP一2transfectedBMSCs+HA(n=8)，II．Adv—hBMP一2transfectedBMSCs(n=8)，III．untransfeetedBMSCs(n=4)，Ⅳ．HA(n=4)．Theosteoinductiv

7、ityinmuscleofnudemicewasinvestigated7圭墨篁三墨型茎兰型!墼堡主塑塞竺堂垡塑byX-rayexam，osteogenesisquantificationandhistologicalexsa]．(3)Therepairofradialshaftdefects(1．5锄)inrabbits：20rabbits(40sides)wererespectivelyinjectedthecellsand／ormaterialsaccordingtothefollowin

8、gfourgroups：IAdv—hBMP-2transfectedBMSCs+HA(n=lO)，IIAdv—hBMP一2transfectedBMSCs(n=lO)，IIIHA(n=lO)，Ⅳcontrol(n=lO)．Roentgenography，histologicalexam，biomechanicaltestandhistomorphometricalanalysiswerestudiedatvarioustimepoints．Results(1)Testinvitro：hBMP一2