鼻咽癌患者血浆eb病毒dna定量检测与其临床意义

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1、鼻咽癌患者血浆EB病毒DNA定量检测及其临床意义中山大学硕士学位论文QuantificationDetectionandClinicalSignificance-蹩薹!耋萋簦辇摹妻鏊蓁耋饕l蚕一霎萋孚塞搿；茎妻墨羹囊糯；粪矬耋i囊薹薹薹薹蠢冀冀錾薹l蓁嚣妻萋l蔷

2、上尽囊型薹喜鎏l零喜萎耋i妻蓁藿藿藏薹!冀薹薹羹lzl塞薹"xVC～IgAalldE～IgA舶m84healthycontmls．2．P1aSmawerecollected舶m149post-treatmentNPCpatientswhoweretreatedinourhospitalbet、vee

3、nDecember2006toDecember2007including60metastasispatients，29locoregionalrecurrencepatlents，60remlsslonpatientswho、verefollo、vedupformorethall2years．Thesepatientswerediagnosedwiththeclinicalradiographicand／orpathologicallyeVidences．TheplasmawerecoIlectedtodetectionEBV—DNA、VCA／IgAandE

4、A／IgA．Amongthe60metastasispatients，PeripheralbloodwaScollectedf硒m16atevenr2cycleschemotherapyfinishedandassessresponsewithradiographic．3．ForEpstein—BallrVirusDNAinplasmawaSdetectedinreal．timequantitativePCRsystem；VCA／IgA、EA／IgAweretestedbyimmunonuorescenceexperiments．4．Inthe1l3new-

5、diagnosiedNPC，61caSeshadtwocyclesneoadjuvantchemotherapyandradiotherapy．52casesonlyhadradiotherapy．Theregimenofneoadjuv觚tchemotherapy、^，asconsistedofCispIatin20mg·(m2·d)。1ond1．d5and5．FU750mg‘(m2‘d)．1，giVenasa5一daycontinuousin向sion．Theradiation、VasadministeredbyC060ybeamsor6-8MVphot

6、onbe锄s．TheradiationportalsencompassedthenasopharynxandadjacentregionsatriskoflocaltumorspreadaJldbothsidesneck．Theprescriptiondosetothenasopha巧nxgrosstumorwas68．74Gy；60Gyto64GythepositiVenecklymphnodes，and50Gyfbrprophylacticradiation，allusingconventionaldosef}actionation．TheSixteen

7、metastasispatientshadsixcycleschemother叩y．TheregimensofchemotherapywerePF、TPF、TP．5．Allstatistic羽analyseswerepe“bnnedwithSPSS13．OsoRware．ThemedianEBV．DNAwerecomparedusingnonparametrictest．Therate(proponion)werecomparedusingchi-squaretest．Atwo-tailedpvalueof

8、callvsignificant．Results：PlasmaEBV二DNAwasdetectablein80％(90／l13)ofprima巧NPC，2．4％(2／84)ofheaIthycontr01．ThemediaJlplaSmaEBV-DNAconcentrationwaS34200copies／ml锄d0copies／ml，respectively．T11edi仃erencebet、)~reentheSet、vogroupsisstatisticaUysignificant⑦20．OOO)．’I’ocomparetheReceiveroprati

9、ngcharacteristic(ROC)cun，e