stat3抑制剂stattic对肝癌细胞生长、放射敏感性和迁移侵袭的影响

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1、万方数据江苏大学硕士学位论文Objective：AbstractTostudytheeffectoftheSignaltransducerandactivatoroftranscription一3(STAT3)inhibitorStattic(Y705)onthegrowth，migration，invasionandradiotherapysensitizationofhepatoeellularcarcinomacelllinesanditspreliminarymechanism．Atthemeantime，toinvestiga

2、tethepotentialapplicationoftheinhibitionofSTAT3ontheradiotherapyoflivercancelinordertodiscovernewtargetforradiotherapysensitization．Methods：1。ToinvestigatethemRNAexpressionlevelsofSTAT3inthreekindsofhepatocellularcarci‘nom’、acelllinesbyRT-PC!R，andtheproteine婶ressionlevel

3、sofSTAT3andp-STAT3inlivercancercellsbyWesternBlot．2．TodetecttheeffectofStatticonthegrowthoflivercancercelllinesSMMC一7721，HepG2，Bel一7402byCCK8method．3．TodetecttheeffectofthecombinationofStatticandX—rayradiationonthegrowthoflivercancercelllinesSMMC一7721，HepG2，Bel一7402byCCK

4、8method．4．ToinvestigatetheinfluenceofStatticonradiationsensitivityoflivercancercelllinesSMMC-7721，HepG2，Bel一7402bycloneformationassay．5．TostudytheeffectsofthecombinationofStatticandX-rayradiationonapoptosis—relatedproteinsinlivercancercelllinesSMMC一7721，Bel一7402byWestern

5、Blot．6．ToinvestigatetheinfluenceofthecombinationofStatticandX—rayradiationonthecellmigrationofBel一7402bythescratchmigrationassay．7．ToinvestigatetheinfluenceofthecombinationofStatticandX-rayradiationonthecellinvasionabilityofBel一7402bytranswellassay．8．Todetecttheexpressio

6、nofinvasion-relatedproteinsinBel-7402withthecombinationofStatticandX-rayradiationbyWesternBlot．Results：1。Inthreekindsoflivercancercells，theexpressionofSTAT3ishigherinSMMC一7721andBel一7402，buttheexpressionofpSTAT3ishighestofBel-7402。2．Statticsignificantlyinhibitthegrowthof

7、humanlivercancercelllines。Inthreekindsoflivercancercell’linesSMMC一7721，HepG2andBel一7402，theIC50ofStatticafter48hrtreatment万方数据STAT3抑制剂Stattic对肝癌细胞生长、放射敏感性和迁移侵袭的影响was5．13uM，2．94uMand2．54uM，respectively．3．ThereisasynergisticeffectbetweenStatticandX—rayradiationontheinhibit

8、ionofthegrowthoflivercancercells．InSMMC一7721．thecellsurvivalrateof8GyX—rayradiationgroupwas62％土5％，thera