胶体金免疫层析法快速定量检测猪尿中β2-受体激动剂残留的分析

《胶体金免疫层析法快速定量检测猪尿中β2-受体激动剂残留的分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、ABSTRACTcarriedouttoexplorethestabilityoftheteststrips.Theresultsshowedthatthestabilityofthecontrollinewasthekeyfactor.Theractopamineteststripshowedbetterstabilitythanclenbuterolandsalbutamolteststrips.ThecontrollinessignificantlydecreasedleadingtoanincreaseofT/Cratio,themainfactoraff

2、ectingthestripstabilitywasthecontrolline.Toimprovethestabilityoftheteststrip,thegoatanti-mouseantibodyspottedonthecontrollinewasmixedwithWellchampion(5%,v/v).Furthermore,thenewlypreparedstripsshowedsatisfactorystabilitybydryingthenitrocellulosemembraneat60℃foroneday.Besides,colloidalg

3、old-basedlateralflowimmunoassayforrapidquantitativedetectionsystemhasbeensuccessfullydeveloped.Thelimitofdetectionofclenbuterol,ractopamineandsalbutamolresiduesinswineurinesampleswere0.19μg/L,0.39μg/L,and0.83μg/L,respectively.Andtherecoveriesofthedetectionsystemrangedfrom86.6%to109.7%

4、withthecoefficientofvariationsbetween4.7%and14.7%.Thelimitofdetectioninporksampleswas0.43μg/kg,0.52μg/kg,and1.01μg/kg,respectively.Theresultsshowedthattherecoverieswereintherangeof71.2%to101.6%withthecoefficientofvariationsrangedfrom7.2%to17.9%,whenthedetectionsystemwasevaluatedbyspik

5、edporksamples.Inthestudy,thequantitativedetectionsystemhasbeenappliedtodetect135swineurinesamplesand710porksamples.Theresultthatallsampleswerenegativeshowedthatclenbuterol,ractopamineandsalbutamolhavebeeneffectivelymonitoredinourcountry.Keywords:β2-agonist;lateralflowimmunoassay;quant

6、itativedetection;stability.IV缩略语表缩略语表(Abbreviations)英文缩写英文全称中文CLEClenbuterol克伦特罗RACRactopamine莱克多巴胺SALSalbutamol沙丁胺醇CLPClorprenaline氯丙那林LFIALateralFlowImmunoassay免疫层析技术HPLCHighperformanceliquidchromatography高效液相色谱法LiquidChromatographyTandemMassLC-MS/MS液相色谱串联质谱法SpectrometryNCNitrocellu

7、lose硝酸纤维素BSABovineSerumAlbumin牛血清白蛋白IC50Half-maxinalInhibitoryConcentration50%抑制浓度SDStandardDeviation标准偏差CVCoefficientofvariation变异系数PBSPhosphate-bufferedsaline磷酸盐缓冲溶液RIARadioimmunoassay放射免疫分析法TRFIATimed-resolvedfluoroimmunoassay时间分析免疫荧光法SFCSupercriticalfluidchromatography超临界流体色谱法FMIA

8、Fluor