实验性大鼠脑缺血后脑红蛋白的动态表达与保护机制分析

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1、英文摘要ExpressionofNeuroglobinandMechanismUnderlyingitsNeuroprotectiveActionafterFocalCerebrallschemiainRatsABSTRACTObjective：Cerebrovasculardisease(CVD)iscommonlyandfrequentlyencountered，endangeringthepeople’Slifeandworkbecauseofitshighmortalityandseriousdisability．一●●●一‘tsereb

2、rallschemiaaccountsforseventypercentsofCVD．Themechanismofbrainedemaformationandsecondaryneuronlnjuryarenotveryclearnow．Uptonow,thereiSnoonekindofmechanismstoexplainthedamagetheoremaftercerebralischemia．Researchersconsiderthatseverealmechanismsparticipatetheprocessofcerebralin

3、farction：metabolicblock，calciumoverload，cellapoptosis，thetoxicactionofexcitatoryaminoacidsandfleeradicle，thenervedamagemediatedbymtrogenmonoxidum，eta1．Hemorrhagicinfarction，asnamedhemorrhagictransformation，isafrequentoutcomeofischemiccerebralinfarction，whichisespeciallysevere

4、afterthrombolyticandanticoagulanttherapy．However，itsspecificmechanismatenotveryclear．Neuroglobin(Ngb)isanewlydiscoveredproteinlocalizedinneuronsofthecentraIandperipheralnervoussystemsinvertebrates．Itfunctionstobind，store，andfacilitatetheutilizationofoxygeninneuronalcells．Rece

5、ntstudiessuggestthatitmaymodulatehypoxicand———————————————————————————————————————～一一S英文摘要ischemicinjury．Ngbcanalsobeinducedbyhemininadose—dependentmanner．BindingassayinvitroconfirmedtheinteractionbetweenNgbandNa+，K+．ATPasebeta2subunit(NKAlb2)．Itissuggestedthathypoxia—induced

6、NgbcouldinhibittheNa+．K十．ATPaseactivitytOcertainextenttoprotecttheneuronfromhypoxicinjury．Thepurposesofthisstudyaretostudytheexpressionofneuroglobinaftermiddlecerebralarteryocclusion(MCAO)andhemorrhagicinfarction(HI)inratsandtheeffectofheminonischemicbraintissues．Methods：200S

7、prague-Dawleyratswererandomlydividedintosham·operatedgroup，MCAOgroup，HIgroupandhemintreatmentgroup．Threelatergroupsweredividedinto4subgroupsrespectivelyaccordingto3一，6·，12-，and24haftertheanimalmodalformation．Alltheratsweretestedforneurologicalbehaviorandthenweresacrificed．The

8、brainwatercontentwascalculatedasthepercentagechangesbetweenwetweight