非离子型表面活性剂复合双水相体系与其蛋白质分配平衡分析

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1、汕头大学硕士学位论文sizewouldaffectthepartitioningbehaviorofmodelproteins.TheresultshowsthattheproteinswithlowmolecularweightsuchasLYSandα-LAtendtoaggregateinPEGphase,thistendencywouldbeenhancedbyincreasingTLLofATPS.However,thepartitioningcoefficientofthehighermolecula

2、rweightproteinsuchasBSAandOVAdecreasedramatically.Moreover,withPEGmolecularweightrising,thepartitioningcoefficientwillreducesignificantly.Additionally,alltheselectedmodelproteinsarepartitionedpreferablyintothesalt-richphase,andthepartitioningcoefficientdecrea

3、sewithincreasingTLLinbothPEG/Na2SO4ATPSandTX-100/Na2SO4ATPS.（4）Thehydrophobicinteractionandexclude-volumeinteractionpartitioningmodelwasappliedtocorrelatethepartitioningcoefficientsofmodelproteins.TheresultsofqualitativeanalysesindicatethatthecontributionofTX

4、-100-richphaseforexclude-volumeinteractioninproteinpartitioningintheaqueoustwo-phasesystemscomposedofPEGandTX-100isstrongerthanPEG-richphase,andthetrendwouldbeenhancedbyincreasingTLLandlowerthePEGmolecularweight.Also,theeffectofhydrophobicinteractionmaketheta

5、rgetproteinpartitiontothebottomphase.HydrophobicresolutionofATPSincreaseswithincreasingTLLandPEGmolecularweightwhilethechangeofintrinsichydrophobicityisnotobvious.（5）ThePEG/TX-100aqueoustwo-phasesystemwasappliedinpartitioningofmembraneproteinseparationofHalob

6、acteriumhalobium.Theseparationofthemembraneproteinweremadebycomparingtheaqueoustwo-phasesystemandsucrosegradientcentrifugation.TheresultdemonstratedthatthetargetproteincouldpartitionintotheTX-100-richphase,andthecontaminantproteinswereextractedintothePEG-rich

7、phasebymulti-stageextractprocess.Keywords:aqueoustwo-phasesystems,polyethyleneglycol,TX-100,exclude-volumeinteraction,hydrophobicityinteractionIV万方数据汕头大学硕士学位论文目录摘要................................................................................................

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