非离子型表面活性剂双水相系统特性与其应用分析

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1、汕头大学硕士学位论文equilibriumofATPScontainingadditivewerecarriedout.TheresultsshowedthatanionicsurfactantSDScouldmakeTritonX-100/Na2SO4ATPSstable.SDSgreatlychangedthetwo-phaseregionandformingpropertyofTritonX-100/Na2SO4ATPS.Whenthetie-linelengthofTritonX-100

2、/Na2SO4ATPScontainingSDSwasintherangeof23.10to30.84,thelongerthetie-linelength,thehigherthemassfractionofSDSatthemidpointoftie-line,themorestablethephaseseparation.(3)Inordertogetanonionicsurfactant-basedATPSwhichwasstabletotemperature,thestudyofTrit

3、onX-114/saltATPSwasalsoconducted.TheeffectofTritonX-114concentration,salinityandionicspeciesonthecloudpointofTritonX-114werealsoinvestigated.TheriseofTritonX-114concentrationcausedmicelleschangedfromquantitytoshape,madethecloudpointfirstlydecreased,t

4、henconstantandlastincreased.Assalinityincreased,moresaltionswouldcompetingforwatermoleculeswithTritonX-114andmadethecloudpointgraduallydecreased.TheinfluenceofMg2+onthecloudpointwasslightlymorethanNa+,andthereducingeffectofSO42-onthecloudpointwasgrea

5、terthanCl-.Therefore,TritonX-114/MgSO4ATPSwaschosenforsubsequentpracticalenrichmentofhydrophobicantibacterialpeptideNisin.(4)TookantibacterialpeptideNisinastheresearchobject,TritonX-114/MgSO4ATPSwasusedtoseparateNisinfromfermentationbrothofLactobacil

6、luslactis(ATCC11454).UtilizedbacteriostaticactivityofNisinobtainedfromATPSastargettooptimizetheenrichmentcondition.Thebestenrichmentconditionwasconcentratingthefermentationbrothsixtimes,andthereaftermixingwithPBS(pH=7.0)ataratioof2:1,thenaddingtoATPS

7、containing4%(w/w)TritonX-114and0.05MMgSO4.Afterachievingphaseequilibrium,thusthesurfactant-richphaseacquiredwasdilutedeighttimes toobtaintheantibiotic(mixtureofantibacterialpeptideNisinandnonionicIV万方数据汕头大学硕士学位论文surfactantTritonX-114)withthehighestti

8、ter.Thebacteriostaticactivityoftheantibioticcouldreach91.43%ofstandardNisin(0.01g/mL)producedbySigma,therecoveryofantibioticpotencywas35.11%.Theresultsofinhibitedeffectoftheantibiotictootherfivebacteriashowedthattheantibiotichadsignificantbacteriosta