双水相溶剂体系的正交轴逆流色谱与其应用分析

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1、汕头大学硕士学位论文band.Moreover,amodifiedcorrelationequationincludingthephaseseparationrate,TLLandphysicalpropertiesoftheATPShasbeenproposedandsuccessfullytestedinthebottom-continuousphasewhosecoefficientswereestimatedthroughregressionanalysis.Theproposedcorr

2、elationequationpredictedthephaseseparationratewithcorrelationindex(R2)of0.960forPEG/ammoniumsulfateATPSand0.971forPEG/sodiumcitrateATPS,respectively.3.Studyonthestationaryphaseretentionofcounter-currentchromatographywithX-axisCPC.Thestationaryphaseret

3、entionhasbeeninvestigatedwithdifferentelutionmode,differentrevolutionanddifferentflowrate.Theresultshows,forthePEG1000/sodiumcitrateATPS,thestationaryphaseretentionis54.83%attherevolutionspeedof800rpmandtheflowrateof0.5ml/min;forthePEG1000/ammoniumsul

4、fateATPS,thestationaryphaseretentionis51.63%attherevolutionspeedof500rpmandtheflowrateof0.3ml/min.4.StudyonthePEG/saltATPSwhichisusedfortheseparationofphycocyanin.ThepartitioncoefficientofphycocyaninhasbeenmeasuredintheATPSwithdifferentweightofPEG,dif

5、ferentTLLanddifferentsalt.TheresultshowsthatthepartitioncoefficientofphycocyanindecreasewiththeincreasingweightofPEG.ThePEG6000/sodiumcitrateandPEG4000/sodiumcitrateATPScanbeusedforcounter-currentchromatographicseparationasthepartitioncoefficientofphy

6、cocyaninis1.00and1.96respectively.Keywords:cross-axiscoilplanetcentrifuge;aqueoustwo-phasesystems;systemproperties;phaseseparationbehavior;stationaryphaseretentionIV万方数据汕头大学硕士学位论文目录摘要....................................................................

7、.............................................................IAbstract........................................................................................................................III目录........................................................

8、......................................................................Ⅴ第一章前言................................................................................................................11.1逆流色谱技术简介...........................................