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ID:33010624
大小:2.60 MB
页数:39页
时间:2019-02-19
《线粒体基因组d-loop区单核苷酸金态性与胃癌发病风险关系的分析》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、英文摘要IdentificationofsequencepolymorphismsintheD—LoopregionofmitochondrialDNAasariskfactorforgastriccancerABSTRACTObjective:Gastriccancerisoneofthemostfrequentcancersintheworldandthreatenspeople’Shealthduetothehighmortalityrate.Recently,itwasnoticedthatthemitochondrialDNA(mtDNA)mu
2、tationandsinglenucleotidepolymorphisms(SNPs)wereassociatedwiththeoccurrenceofcancers.ThehumanmtDNA,astheonlygeneticmaterialofextranuclear,isa16569bp,circularanddouble—strandedDNAcodingfor13polypeptidesinvolvedinrespiratorychaincomplexes,aswellas22transferRNAsand2ribosomalRNAsinvo
3、lvedinproteinsynthesisinmitochondria.Thedisplacementloop(D—Loop)istheonlynon-codingmtDNAregionwhichisimportantforregulatingbothreplicationandexpressionofmitochondrialgenomebecauseitcontainstheleading—strandoriginofreplicationandthemainpromoterfortranscription.Ithasbeenreposedthat
4、theD—Loopregionisahotspotformutationsandpolymorphismsindifferenttypesofcancers,includinggastriccancer.SNPsisthepolymorphismsinducedbysinglenucleotidevariationintheDNAsequence,whichhasbeenwidelyappliedtoresearchsusceptiblegenesofmultigenicdisease.Inthisstudy,theD-LoopregionofmtDNA
5、wassequencedonaseriesof145gastriccancerpatientsand159heathycontrols,therelationshipbetweenthegastriccancerrisk-associatedSNPsandclinicalfeatureswasevaluated,andthepotentialroleofmtDNApolymorphismsinoccurrenceandprogressionoftumorswasexplored,whichwouldbehelpfultodetectvaluablemar
6、kersforgastriccancerriskevaluationandmaketherapeuticdecisionsearlier.Methods:1Objectofstudyandsamplecollection:Bloodsampleswerecollectedfrom145gastriccancerpatientswhounderwentgastriccancerresectioninthedepartmentofgeneralsurgeryattheFourthHospitalofHebeiUniversityfrom4英文摘要2007to
7、2008.Allpatientswerediagnosedhistopathologicallybeforeoperaion.Bloodsampleswerealsocollectedfrom159healthycontrolswithoutafamilyhistoryofgastriccancerwhowerechecked.upinthesamehospital.Informedconsentswereobtainedfromallparticipantspriortoenrollmentandallthesampleswerecollectedac
8、cordingtotheHumanTissueResearchCommittee
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