酪氨酸激酶etk2fbmx对鼻咽癌放射敏感性影响的分析

《酪氨酸激酶etk2fbmx对鼻咽癌放射敏感性影响的分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、ABSTRACTin1972，moreapoptosiscontrollinggenesuchasbcl一2，p53etalhavebeenknownbyhumans．Inaword，peoplehavebeenfoundouttheapoptosismechanismofNPCmoreandmore，especiallytheapoptosiscontrollinggene．Theendothelial／epithelialtyrosinekinase(Etk／BMX)isoneofthenon·receptorphosphatetyrosineki

2、nasefamiliesofBtk．Itwasfoundinprostaticcarcinomacells，andidentifiedinhumanbonemarrowcell，SOitisalsocalledbonemarrowXkin-ase(BMX)．ItislocatedinchromosomalbandXp22．2betweentheDXS197andDXS207loci．BMXeDNAconsistsofalongopenreadingframeof675aminoacids，containingastructureresemblingth

3、eBtkfamilykinasepleckstrinhomologydomain(PH)，anSH3(Srchomology)domain，anSH2(Srchomology2)domain，andaca-talytickinasedomain．Foreignscientistinprostaticcarcinomaandbreastcancerresearchhavebeenfoundthat：Etk／BMXplayedanimportantroleincarcinomacellsfromXrayirradiation．Interestingly,i

4、tisfoundthatEtkexpressesabnormallyinnasopharyngealcarcinoma，SOweusethetransfecedcelllinesune—wthighlyexpressingEtktostudytherelationshipbetweenthetyrosinekinasesEtkandradiosensitivityinvivoandvitroinnasopharyngealcarcinoma(NPC)．objeetive：Tostudytherelationshipbetweenthetyrosinek

5、inasesEtk／BMXandradiosensi—tiveityinnasopharyngealcarcinoma(NPC)．Method：ByusingLipofectamine2000(Invitrogen)followingthemanufacturer'sins-truction，recombinantplasmidpcDNA3．0一Etkatetransfectedintosune-1，andEtkishi曲lyexpressedinthetransfectedcelllinesane·wt．After0，2，4，10，15GyXrayr

6、adiation，MTTisusedtomeasuresurvivalfraction(SF)，andflowcytometer(FCM)硕士学位论文assaytoexaminecellapoptosisrate，cellcycle，proliferationindex(PI)，andapoptosis-relatedproteinp53，bcl-2，bcl—xlandbak．sane一1，sane—wtandsune。vectorarcinjectdsubcutaneously(s．C)intonudemice(BALB／c，nu／nu，4-6wee

7、ks，lSg)．Ani。malsarerandomizedfortherapyin21days(n=7ineachgroup)．TumorvolumearedeterminedthreetimesweeksbydirectmeasurementwitllslidingcaliperandcalculatedbytheformulavolumeV=(4／3)×xxRlzxR2，whereRIisradius1andREisradius2andRl

8、acceleratorasdescribedpreviouslytotumorstarting