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《rhpth(1-34)对兔下颌骨牵张成骨作用的实验分析-(5349)》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、AbstractEffectofrhPth(1-34)WalkeronmandibulardistractionosteogenesisinrabbitsTheFourthAffiliatedHospitalofZhengzhouUniversityAbstractObjective：TostudytheeffectofrhPTH(1-34)onpromotingboneformationonmandibulardistractionosteogenesis（DO）inrabbits,soastoprovidefoundationfori
2、tsclinicalapplicationofrhPTH(1-34)inthefieldofDO.MaterialandMethods：8big-earedJapaneseRabbits（nosexlimit,weightsabout2.0-2.5Kg）wereselectedasexperimentalanimals,whichwererandomlydividedintothecontrolgroup,therhPTH(1-34)12.5μg/kggroup,therhPTH(1-34)25μg/kggroup,andtherhPTH
3、(1-34)50μg/kggroup,withtenrabbitsineachgroup.Theirpartsaheadnexttopremolarteethandmentalforamenareaontherightsideofthemandibularswereslitedinordertoestablishunilateralmandibulardistractionosteogenesismodels.Distractionwasstartedoneweekaftersurgeryattherateof0.4mm/12h,whic
4、hcontinuedfor10days,extendingmandibularby8.0mmandfollowedbyfixedperiod.ThecorrespondingdoseofrhPTH(1-34),dissolvedin2%heatinactivatedstandardrabbitserum1ml,wasgivenateighteverydayviasubcutaneousinjectiontotheexperimentalgroupsfromthedistractionperiodtilltherabbits’deathwh
5、liethecontrolgroupwasonlygiven2%heatinactivatedstandardrabbitserum1mlatthesametimeeveryday.Fiverabbitsfromeachgroupwereexecutedatthefirstweekandthirdweekinfixedperiodrespectively.Thenthenewbonesindistractionregionwereselectedasthespecimens.Thegeneralobservation,histologyo
6、fHEstainingandimmunohistologyofBMPsexaminationwereperformedwiththeresultsisticalanalyzedbythestatisticalsoftwareSPSS17.0andtheheart,liverandrenalremovedIII万方数据Abstractforroutinepathologicexaminationrespectively.Result：1.Thedistractionosteogenesismodelswereestablishedsucce
7、ssfully.Noneoftherabbitsweredead.Therightsideofmandibularextendsby8.0mm,andocclusaldisordercouldbeobservedineveryrabbit.2．Generalobservation:Inthefirstweekofthefixedperiod,newbonetissuesoftheexperimentalgroupswerebetterthanthoseofthecontrolgroup.Asthedoseincreased,newbone
8、becamebetter.Inthethirdweekofthefixedperiod,everygroupobtainedahardtexture,withthetextureofexper
