cytc信号转导对胆道梗阻大鼠中性粒细胞凋亡调控分析

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1、广州医科大学硕士学位论文Cytc信号转导对胆道梗阻大鼠中性粒细胞凋亡调控研究PMN较A和B组减少，D组凋亡PMN较C组增多。PMN凋亡率与ΔΨm的变化呈负相关，PMN凋亡率与Caspase-9、Caspase-3的活性均呈正相关，PMN凋亡率、Caspase-3的活性与Caspase-8的活性无相关性。【结论】大鼠外周血PMN凋亡减少贯穿胆道梗阻的病理过程。PMNΔΨm的稳定，阻碍了线粒体内Cytc向细胞质内释放，导致内源性凋亡通路下游信号分子Caspase-9、Caspase-3激活减少，进而影响胆道梗阻大鼠PMN凋亡进程。外源性凋亡通路信号分子Caspase-8未参

2、与胆道梗阻PMN凋亡的调控。外源性Cytc可促进胆道梗阻PMN凋亡，其机制可能与激活线粒体Cytc信号转导通路有关。【关键词】胆道梗阻；中性粒细胞；凋亡；线粒体；细胞色素c；半胱天冬酶-3。2万方数据英文摘要ExperimentalstudyoftheregulationofCytcsignaltransductionpathwayonneutrophilsapoptosisinbileductligatedratsMasterGraduate：TongmingDengTutor：Prof.YongNiABSTRACT【Objective】Toexplorethecha

3、ngesofpolymorphonuclearneutrophils(PMN)apoptosisinbileductligatedrats,andstudytherolesofcytochromec(Cytc)signaltransductionpathwayonPMNapoptosisinbileductligatedrats.【Methods】104SDratswererandomlydividedintofourgroupsasfollows:normalgroup(groupA),shamgroup(groupB),bileductligated(BDL)gro

4、up(groupC)andBDLplusCytcgroup(groupD).GroupAtermedas0timepoint,andothergroupswerefurtherdividedintofourtimepointsof12h,24h,48hand72hrespectivelyafteroperation,eachtimepointhad8rats.Bloodspecimenswerecollectedonthetimepointsafteroperation.Liverfunctionandbloodroutineexaminationweremeasure

5、d.PMNwereseparatedwiththemethodofgradientcentrifugationandredbloodcelllysis.PMNapoptosisandmitochondrialmembranepotential(ΔΨm)ofPMNwereanalyzedbyflowcytometry.Cytcproteinexpressionwasdeterminedwithwesternblot.Expressionofcaspase-8,caspase-9,caspase-3activityweremeasuredwithspectrophotome

6、try.MitochondriaofPMNwereobservedbyusingtransmissionelectronmicroscope.【Results】3万方数据广州医科大学硕士学位论文Cytc信号转导对胆道梗阻大鼠中性粒细胞凋亡调控研究PMNapoptosis,expressionofcaspase-9andcaspase-3inratsweregraduallydecreasedfromthe12hto72hafterBDL,whichweresignificantlylowerthanthatinnormalgroupandcorrespondingtim

7、epointofshamgroup.ComparedwithBDLgroup,thereweregraduallyturnhigherthanthatinBDLplusCytcgrouprespectively.ΔΨmofPMNwasincreasedfromthe12hto72hafterBDL,whichhadsignificantchangescomparedwithnormalandshamgroup.ΔΨmofPMNinratsofBDLplusCytcgroup,waslowerthanthatinBDLratsrespect