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ID:32737529
大小:6.41 MB
页数:52页
时间:2019-02-15
《小细胞肺癌外周血和组织中耐药基因表达检测》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、者外周血淋巴细胞MDRlmRNA和MRPmRNA的表达水平增强;免疫组化结果显示P.gP,MRP,TOPO-1I在SCLC中均有不同水平表达,它们的表达对SCLC的耐药起重要作用,因此检测这三种耐药基因有助于判断化疗疗效及预后;在无法获得SCLC肿瘤组织情况下,通过抽取外周静脉血检测MDRlmRNA和MRPmRNA的表达来预测化疗疗效是可行的。硕士研究生傅兴宁(病理学)指导教师陈桦主任医师关键词:小细胞肺癌;外周血;耐药基因;逆转录聚合酶链反应法;免疫组织化学技术Detectionoftheexpressionofre
2、sistancegenesinsmallcelllungcancerAbstractSmallcelllungcancer(SCLC)accountsfor15%-20%ofalllungcancers,whichoriginsfromtheneuroendocrinefeaturesofendodermcells.Itisahighlymalignanttumor,withthecharacteristicsofrapidgrowthandearlymetastasis.Inadditiontolittleearly
3、SCLC,chemotherapyandradiotherapywereusuallyinvolvedinmedicaltreatments.Inrecentyears,despitegreatprogressesachievedincombinedtreatmentmeasuresincludingchangesofdrugcombination,drugdosesortimeofoperation,clinicaltherapeuticeffectisstillnotsatisfactory.Multidrugre
4、sistance(MDR)isthemainreasonresponsibleforthefailureofchemotherapyandreplaseoftheSCLC.SomestudiessuggestMDRphenomemonintumorcellsiscloselyrelatedwithoverexpressionofMDR1andMRPgenes.However,idealreversaldrugstargettingforMDRisstillunderresearch.objeet:Todetermine
5、thesignificanceofMDR1gene、MRPgeneTOPO一11geneexpressioninperipheralbloodlymphocytes(PBMCs)ofSCLCpatientsbeforeandafterchemotherapy,andexploretheproteinlevelofP—gP、MRP、TOPO—IIinSCLCtumortissues,aswellasstudythecorrelationofMDRl、MRP、andTOPO—IIexpressioninPBMCsandtu
6、mortissues.Methods:RT-PCRmethodandimmunohistochemistrymethodwereusedinthepresentstudy.Results:1.Detectingthepherepheralbood,theexpressionlevelsoftheMDRlmRNAandMRPmRNAinPBMCsinSCLCgroupwerehi曲erthanthatinthecontrolgroup(P<0.05).TheexpressionlevelsofMDR1mRNAandMRP
7、mRNAafterchemotherapyinSCLCweresignificantlyhigherthanbeforechemotherapy(P<0.05,O.001).2.TheexpressionlevelsofMDR1mRNAandMRPmRNAinextensivestageweresignificantlyhigherthaninlimitedstageOfSCLCafterthreecyclesofchemotherapyfP<0.05).Nocorrelationswerefoundbetweenth
8、eexpressionlevelsofMDR1mRNAandMRPmRNAinPBMCsandage,gender,smokinghistory,tumorsize,clinicalstagesandlymphnodemetastasis(P>0.05).3.Intumortissues.thepositiveratesofP-g
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