富血小板血浆对兔半月板无血管区损伤修复的作用

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1、英文摘要1，1、t一··一．一．1工herewerenopostoperativecomplicationssuchasinfectionhappened．2TheplateletconcentrationinPRPextractedbytwo—stepcentrifugationmethodwasas4．68timesasinwholeblood，whichcanconformtotherequirementsofPRPonplateletcontent．3GeneralobservationInexperimentalgroupingroupA，therewasthinfilm

2、—liketissuegrowbetweentheinjurycrackbuttheinjurycrackwasstillobvious．IngroupB，theinjurycracksofexperimentalmeniscuseswerepartlyfilledwithfibrousscar-liketissue，andtherewerenoobviouscracks．ⅥmileingroupC．fibrillarconnectivetissuefullyfilledinthecracksofmeniscusesinexperimentalgroup．Incontrolgrou

3、p，theinjurycracksofmeniscuseswereobvioustill5weekspostoperatively．SomemeniscusingroupC，therecouldbeseenthatthecrackswerepartlyfilledwithabitoffibrousscar-liketissue．Statisticalanalysisresultsshowedthatthehealingconditionofexperimentalgroupwassignificantlybetterthancontrolgroup俨

4、pA，BandC．4HistologicalobservationInexperimentalgroupofgroupA，therewerelotsofcells．whichwas／inthemajoritywithinflammatorycellsandfibroblast，infiltratedintheedgeofmeniscus岫urycracks．Andtherewerelittlefibro．chondrocyteappeared．ThereparativetissuewasnotobviousstainedwithSafranin．0．WhileingroupBand

5、C，reparativetissuefilledintheinjurycracksgraduallyincreasedandwascloselyconnectedwithsurroundingnormalmeniscustissue．Fibro—chondrocyteandextracellularmatrixincreasedgradually．AndinECM，fibrillationandneovascularizationcouldbeseen，thetissuestailledwithSafranin．Oalsoexpandedgraduallywhichmeanthat

6、thecompositionofcartilageincreasedgradually．IncontrolgroupofgroupA，theedgeofmeniscusinjurycrackswerecoarseandneitherinfiltratedcellsnorfibro．chondrocytescouldbefound．5英文摘要IngroupBandC，reparativetissuepartlyfilledintheinjurycracksbutnotcloselyconnectedwithsurroundingnormalmeniscustissue．Thenumb

7、eroffibro‘chondrocytesandECMwerelessthanwhichinexperimentalgroupandfibrillationandneovascularizationdidnotappearinthereparativetissue．Statisticalanalysisresultsshowedthatthehealingconditionofexperimentalgroupwassignificantlybetterthanco