多西环素对哮喘大鼠vegf、mmp-9的作用及血管重塑的影响

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1、英文摘要eosinophilsintheBALFwere．counted．LungtissueswereslicedandstainedwithHE．TheexpressionsofVEGFandMMP-9inlungtissueswereobservedbyimmunohistochemistrycombinedwiththemicro—imageanalysis，andmeanopticaldensitiesofVEGFandMMP-9weremeasuredbyimageanalysissotbecare．ThecontentsofVEGFinserumwerem

2、easuredbyELISA．Themakerssuchastheairwaywallthicknessandvasculardensityweremeasuredbyimageanalysissoftware．Results：1ResultsoftotalcellsandeosinophilsintheBALF(xl06)：ThenumbersoftotalcellsandeosinophilsintheBALFinthethreegroupswereasfollows：Controlgroupwere9．42士3．67，1．24+0．72．Asthmagroupwe

3、re35．53+7．06，4．84士0．65．Doxycyclineinterventiongroupwere14．66士5．81，2．14士0．58．Theasthmagroupweresignificantlymorethanthedoxycyclineinterventiongroup(P<0．01)，andallthecellsweresignificantlymorethanthatinthecontrolgroup(F=64．840P

4、orycellsinfiltration，gobletcellsandsmoothmusclecellsproliferation，vascularhyperplasia,airwaywallthicknesswereobservedintheasthmagroup．Thepathologicalchangesinthelungtissueofdoxycyclineinterventiongroupwereallbetterthantheasthmagroupandwerenotobservedinthecontrolgroup．3Resultsofimageanaly

5、sis：Theairwaywallthickness(rtrn2／rma)andvasculardensity(A／mm2)intheasthmagroup(109．95士13．85，155．55士14．31)weresignificantlymorethanthedoxycyclineinterventiongroup(86．37士5．85，116．64士7．33)衅0．01)．Theairwaywallthicknessandvasculardensityinthetwogroupsweresignificantlymorethanthecontrolgroup(6

6、5．47士7．22，95．45士7．19)(F=58．737P

7、fthetwogroupswerealsosignificantlyhigherthanthecontrolgroup英文摘要(O．24+0．06，0．21士0．04)(F=57．966P<0．01，F=75．261P<0．01)．5ResultofELISA：ThecontentofVEGFinserumoftheasthmagroup(202．86士16．90)wassignificantlyhigherthanthedoxycyclineinterventiongroup(169．94土16．65)(尸<0．01)．Theconte