胃癌横结肠系膜转移的检测及相关因素的研究

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1、山东大学硕士学位论文3．结果表明，虽然常规HE并不能发现，实际上已经有56．7％的胃癌患者发生了横结肠系膜的微转移。由此可以看出胃癌手术清扫横结肠系膜的重要性和必要性。关键词：微转移；胃癌；腹膜：CKl9；CK20。3山东大学硕士学位论文InvestigationoftheDetectionandCorrelationFactorsofTransverseMesocolonMicrometestasisinGastricCancerPostgaraduateWangYanminTutorProf．HeQingsiSpec

2、ialityGeneralSurgeryABSTRACTBackground：Gastriccancerisacommonmalignantoncoma，ofwhichtheannualmortalitylocatesthefirstplaceinallkindsofmalignanttumor．Manysufferersdieofrecurrenceevenafterradicaloperation，becausemicrometastasisesoctet．Micrometastasisismicroscopicre

3、sidualtumorcelldetectedintissue，bodyfluidandcorpusculartransplant．Itexistsoutofprimaryaffectionandcannotbedetectedbyroutineclinicalpathologicalmethods．Currentresearchesconsiderthat，generallyspeaking，clinicalmetastasisisdevelopedfrommicrometastasis．Recently,therap

4、iddevelopmentofhistochemistryandmolecularbiologicaltechniquepromotesthedevelopmentofmicrometastasis．Accompaniedbythedevelopmentandapplicationofimmunohistochemistryandreversetranscription-polymerasechainreaction(RT-PCR)，itispossibleforgastricmicrometastasistobedet

5、ected，andtherealmofthedetectionofmicrometastasisisincreasinglyattractingattention．Themostcommonrecurrenceofadvancedgastriccancerisperitoneummetastasis．Atpresent，therearemanyreportsaboutdetectingperitoneummicrometastasis．Peritoneallavagecytology(PLC)isthemostpopul

6、arandimportantmethodtodetectexfoliatedcancercells(ECC)inabdominalcavityandtoprognose4山东大学硕士学位论文peritoneummicrometastasis．However,PLCalsohasitsdisadvantagethatithasalowsensitivityaboutdetectingsmallvolumecancercell．Ithasahigherrateofmisseddiagnosis，foritspositiver

7、ateisonly14to21percent．Objective：Inthepresentstudy,peritoneumbiopsywasundertakenintraoperatively,cytokeratin(cK)19，CK20immunohistochemistrystainingwereperformedonthetissuefrombiopsy,inordertoinvestigatetheconditionofperitoneummetastasisingastriccancer．Weaimedtode

8、tectthefactorsandrulesofperitoneummicrometastasis，accordingly,toaccuratelydeterminetheclinicalstagingofpatientssufferingfromgastriccarcinoma．Itwouldprovidemore