大鼠脂肪源干细胞的分离培养鉴定及myocardin基因重组慢病毒载体的构建

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1、硕士学位论文Isolation，culture，andidentificationofrats，adilderived。cells(ADSC’)andadiposederivedstemcells(ADbUSJconstructionoflentiviralvectorcarBACKGRoUNDratMyocardingeneName：WuZiyunSul；rvisor：We。AnyangSupervisor：Wel-yangAn●r弋flngErectiledysfunction(ED)isakindofcommondis

2、easeofAndrology,theincreaseofageandbasicdiseasesincreasedsignificantlyhigherriskofED．DiabetesandEDhavebeenprovedtobehighlyrelated．DiabetesgroupshappenEDprobabilityis3timesofdiabeticpopulation，incidenceof35％to90％．ThepathogenesisofdiabeticEDisacomplicatedprocess，Soth

3、erehasagreatdifficultyinthetreatment，asignificantportionofpatientswithdiabeticEDisnotsensitivetofirst-linedrugs(PDEs)，andunableorunwillingtoacceptsecond-lineandthird-linetreatment．Thus，exploringsomerelatedfactorsbasingontheabnormalpathophysiologywillbeanewideafortr

4、eatmentofED．Corpuscavernosumsmoothmuscle(CCSM)isthestructuralbasisofcavernousspacerelaxingandpenileerection，whichplaysakeyroleinthechangeofhemodynamicswhenthepeniserects．Studieshaveshownthatcorpuscavemosumsmoothmuscleapoptosisincreasesandproliferationrateisreducedi

5、ndiabetic，SOthenumberofcorpuscavemosumsmoothmusclecellsdecreasedsignificantly,cellultrastructurepatholo西calchanges，ABS1RACTdecreasedcorpuscavemosumtissuecompliance，triggerED．Therefore，toincreasethenumberofdiabetespatientswimcorpuscavemosumsmoothmusclecellsandrepair

6、cellsfunctionandimprovethefunctionofcorpuscavemosumtissuesmaybeanimportantwaytoimprovethelevelofpreventionandcureofdiabeticED．Similartovascularsmoothmusclecellsaccordingtostructureandfunction，corpuscavernosumsmoothmuscle(CCSM)cellsCanbefurtherdividedintotwotypes：th

7、econtractiontypeandsynthetictype．Themajorfunctionoftheformeroneiscontraction，andthatofthelatteroneisproliferation，migration，secretionandregulationofextracellularproteins，ctc．ThecontractiontypevascularSMCCantransformintosynthetictypeonesandgettobcproliferationwhenth

8、evascularisinjuredorthevascularSMCculturediIlvitroarestimulatedbythegrowthfactors．Thereisastructureofsinusoidinthecorpuscavernosum，whichisverysim