半相合基因效应性t淋巴细胞治疗宫颈癌的小鼠模型研究

《半相合基因效应性t淋巴细胞治疗宫颈癌的小鼠模型研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、英文摘要supernatantofthemediumwasanalyzedbythelactatedehydrogenasekit，andcalculatedtogetthekillingrates．5Thetreatmentindifferentgroups：TheU14一carryingmiceweredivided‘3groupswith8‘‘eachgroup．Controlgroup(Agroup)：PBSinto3groS8miceineachRroControlKrouo2rouv)：PBSwas．fAinjectedbyveinfor0．5ml，e

2、very5daysonce，repeated3times．AutologouseffectorTcellsgroup(Bgroup)：effectorTcellsofC57BL／6micewereinjectedbyveinfor1xl06per0．5ml，every5daysonce，repeated3times．Semi-allogeneiceffectorTcellsgroup(Cgroup)：effectorTcellsofCB6F1micewereinjectedbyveinfor1xl06per0．5ml，everyfivedaysonce，repea

3、ted3times．Calculatedthesurvivalperiodofdifferentgroups．6ImmunohistochemistrymethodwasusedtodetecttheexpressionofSTAT3，P—STAT3，TGF-pl，andIFN-gammaoftumortissueindifferentgroups，theircontentwereexpressedbythepercentageofthepositivecells，whichwereanalyzedbytheimageanalysissoftware．7Obser

4、vingtumortissueandpathologicalchangesofliver，smallintestineinmiceinHEstaining．Results：1ThetimeofsettingupU14一carryingmicemodelWasabout7days．2Thematuredendriticcellsweresuspending，havingdendriticprominen—cies，thepurityoftheeellsurfacemoleculesCDllc+MHCii+was92．4％，andtheCDllc+CD86+was88

5、．8％．3CytotoxicityofeffectorTcellsinvitro：wheneffector-targetratiowas50：1，thekillingratesbetweentheC57BL／6andCB6F1effectorlymphocyteshadnostatisticallysignificantdifferences∽0．05)．WhenthatratioWas25：1，thekillingratesbetweentheC57BL／6andCB6F1lymphocytesalsohadnostatisticallysignificantd

6、ifferences∽0．05)．4Thesurvivalperiodofdifferentgroups：AandBgroups，AandCgroups，bothhadstatisticaldifference衅O．05)．BandCgroupshadnostatisticallysignificantdifference(尸>0．05)．5Theresultsofimmunohistochemistry：ExpressionsofTGF-pl，IFN-7STAT3，andP—STAT3betweenAgroupandCgrouphadstatisticaldif

7、ference英文摘要(氏0．05)．ExpressionsofTGF一131andP—STAT3betweenAgroupandBgrouphadstatisticaldifference衅0．05)，IFN-1'andSTAT3betweenAgroupandBgrouphadnostatisticallysignificantdifferencepO．05)．ExpressionsofTGF—pl，P-STAT3andIFN-7betweenBgroupandCgrouphadstatisticaldifference(P<0．05)，STAT3betwee

8、nBgro