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1、华中科技大学硕士学位论文Studyonthedetectionandapplicationofrenin-angiotensin-aldosteronesystemMasterCandidate:ZhangQiong-fangSupervisor:Prof.ShuBai-huaABSTRACTObjective:DeterminationofreninactivityandplasmaaldosteronearewidelyusedinthediseasesofRenin-angiotensin-aldosteronesystemdisorders,butplasmar
2、eninandaldosteroneconcentrationswerevulnerabletophysiological,pharmacological,andotherpre-analyticalfactors.Somescholarssuggestedthatthescreeningofindividualdiet,timeofspecimencollection,position,anddetectionmethodsofplasmareninactivityandaldosteroneshouldbestandardlizedtoimprovescreenin
3、gaccuracy.Inthisstudy,thelevelsofreninactivityandplasmaaldosteroneinhealthypeopleandpatientswithhypertensionweremeasuredwithRadioimmunoassay.Threeproblemsfurtherlydiscussedinthispaperwere:angiotensinIandangiotensinⅡconcentrationcorrelation,plasmaaldosterone/reninactivityratiowithdifferen
4、tpositioninpatientswithhypertensionandtheestablishmentofseatreninactivityandplasmaaldosteronemedicalreferencerange.Methods:volunteerswerepatientswithhighbloodpressurefromendocrinedivision,urology,andheartdiseaseintongjihospital.Somewereaskedtodoposturesexperiment,whileangiotensinIandangi
5、otensinⅡin180cases.Randomsamplingmethod,medicalgroupsinWuhanQiaokourandomlyselectedthehealthyvolunteersas4华中科技大学硕士学位论文subjects.Theselectioncriterionwasnohistoryofhypertension,andendocrineandmetabolicdiseases.BloodpressurescreeningcriteriaofhypertensionwasaccordingtotheEuropeanclassificat
6、ionstandardin2007(ESC/ESH2007).Patientswithhighbloodpressurelayfastinginthemorningforextractionvenousblood,after4hstandingforerectbloodsampling.Astohealthysubjects,venousbloodwascollecttedinthesittingandrestingstateatleast30min,andthenrapidlycooledinanicewaterbath.After-1plasmaseparation
7、at4℃,2500rmincentrifuge5min,thesamplewereplacedinsealedstorage-20℃.Thebloodsamplescollectedwouldtobemeasuredbyradioimmunoassay.Results:AstheplasmaconcentrationofAngⅠandAngⅡbothshowednon-normalitydistribution,Pearsoncorrelationwasselectedtodescribethecorrelationbetweenthem
