il1β对脑微血管内皮细胞缺氧复氧模型的促血管生成作用研究

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1、ceIrtainhourssoastoestablishhypoxia．reoxygenationmodels．(4)TostudytheeffectofIL．1Binducedbyhypoxia—reoxygenationinjuryonexpressionofVEGFandMMP．9．amonoclonalneutralizingantibodyagainstIL-1pwasusedinthlsexperiment．(5)InordertoinvestigatetheeffectofexogenousIL-1ponculturedBMECtreatedwithhypoxia—r

2、eoxygenationinjury．therewere3groupsinexperiment：controlgroup，hypoxia-reoxygenationgroupandhypoxia。reoxygenatlongrouPwlthIL．1B．SupernatantconcentrationofVEGFandMMP-9proteinswasmeasuredbYenzvme．1inl(edimmunosorbentassayandcellularlevelofmRNAexpressionofVEGFandMMP．9wasdeterminedbyreal-timefluores

3、centquantitativePCR(QRT-PCR)．(6)Athree．dimensionalculturesystemwasestablishedtoobservether01eofexogenousIL．1pontubule．1ikestructureintubeformationassayofBMECtreatedwithhypoxia．．reoxygenationinjury．Results(1)ThepurityandyieldofBMECwassatisfiedwiththefollowingexperiment·TheliabilityofBMECreached

4、thetopofthecellgrowthcurve120hoursafterthebeginningofcultivation．(2)AMTTassayshowed1ng／mlofIL-19reducedBMECviabilitv12hoursafteradministration．(3)SupematantVEGFandMMP-9concentrationsweresignificantlyinducedbyO．1ng／mlIL一19inBMECtreatedwithhvpoxiafor4hoursfollowedbyreoxygenationfor72hours．(4)0．1

5、mg／mlIL一1pelevatedVEGFmRNAlevelsby1．8．foldsandMMP-9mRNAlevelsby2．0_foldsinBMECcellstreatedbyhypoxiafor4hoursfollowedbyreoxygenationfor48hours(5)AfteradditionofmonoclonalneutralizingantibodyagainstIL一1p，nosignificantchangewasfbundinsupernatantVEGForMMP-9levelsinBMECculturestreatedwithhypoxiafor

6、4hoursfollowedbyreoxygenationfor72hours．(6)Tubule‘likestructuresweremarkedlyinducedbyO．1ng／mlIL一19inBMECcellstreatedwithhypoxiafor4hoursfollowedbyreoxygenationfor72hoursConelusionsInsummarv，thisstudyrevealedexogenousIL一19canupregulateVEGFandMMP．9expressionandimproveformationoftubule-likestruct

7、uresinBMECmodelstreatedwithhypoxia-reoxygenationinjury．、，ITTKeyw。rds：interleukin-1p；hypoxia-reoxYgenation；brainmicrovascularend。thelialcell；angiogenesisIX杭州师范大学硕士学位论文IL一1B对脑微血管内皮细胞缺氧复氧模型的促血管生成作用研究英文缩略词ⅧF·AgⅧfactorrelatedantigen第八因子相关抗原B