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1、DAXXinhibitvascularsmoothmusclecellsproliferationandmechanisminballoon-injuredratcarotidarteryABSTRACTObjective:TheeffectofDAXXinhibitsmoothmusclecellsproliferationinballoon-injuredratcarotidarterywereinvestigated,theaimofthepresentwastostudythemechanismsunderly
2、ingit.thusprovideawaytotreatvascularstenosis.Methods:52malerats(400g±100g)wereobtainedfromtheAnimalCenteroftheUinversityofSouthChinatobuiltthestonsismodelofballoon-injuredcarotidarterybyusingliposometopicaltransfectionDAXXplasmid.Werandomlydividedtheminto4groups
3、:sham-surgerygroup、surgerygroup、blank-transfectedgroupandtransfectedgroup.Wetookballoon-injuredcarotidarteryspecimens(30min,1h,2h,6h,24haferthesurgery)forImmunohistochemicaltodeterminethetime-depentmentofDAXXandJNK;4balloon-injuredcarotidarteryspecimensofeachgro
4、upsat7daysand14daysweretakenforHEtoassessmentofthedegreeofvasularrestenosis,forImmunohistochemicaltodetecttheexpressionofprotein,andforPCRtoanalyzetheexpressionofmRNA.Results:DAXXplasmidwastopicaltransfectedinBalloon-injuredratcarotidarterysat7daysand14days,then
5、usingPCRandImmunohistochemical.Fromtheresults,weknewthatDAXXinthetransfectedgroupexpressedmorethaninsurgerygroup,whichsuggestDAXXwassuccussfullytransfectedinthesmoothmusclecells.HEstainingoftissuesliceshowed:comparedwithsham-surgerygroup,smoothmusclecellsinsurge
6、rygroupwassingnificantlyincreased,vesselswallthickening,luminalnarrowing;what’smore,proliferationmoreobviouslyat14day,thatimpliedthemodelwasconstructedsuccessfully;Thesameasblank-transfectedgroup;Comparedwithsurgerygroup,smoothmusclecellsdecreased,luminalwasrela
7、tivelysmooth.Accordingtotheintimal/medicalarearatio,transfectedgroupwassingificantlylower,whichdemonstratedthatDAXXcaninhibitvascularsmooth4万方数据musclecellsproliferationandcaneffectivelytreatvascularstenosis;PCRwasusedtoanalyzetheexpressionofmRNAofDAXXandJNK,itsh
8、owedthatmRNAofDAXXandJNKinsmoothmusclecellsofsurgerygroupexpressmorethansham-surgerygroup,itsuggestedsurgerycouldpromotetheexpressionofDAXXandJNK;AndtheexpressionofDA
