grim-19在小鼠植入前胚胎中表达及作用的研究

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1、山东人学颂：t：学位论义一个潜在指标。3、GRIM．19蛋白的阻断能影响胚胎的存活率，并阻滞小鼠早期胚胎的发育，提示GRIM．19在胚胎的早期发育中发挥重要作用。关键词：GRIM．19；线粒体；植入前胚胎；Westernblot；Real—timePCR；显微注射山东人学硕．L

2、学位论文ThestudiesonexpressionofGRIM一19inpreimplantationembryo。’miceLnditsfunctionemrOOtmlceanItSUncSpeciality：Gynecologyand

3、ObstetricsPostgraduate：CuiWenjuanSupervisor：Prof．DengXiaohuiProf．ChaoLanABSTRACTPurposesMitochondriaaretherichestoforganellesineggcytoplasm，whicharethemajorsourceofATPgenerationforegg，fertilizedovumandembryo．Duringtheearlydevelopmentofembryo，mitochondriaunderg

4、oaseriesofchangesinmorphologyandlocation．Duringthisstage，littlechangeinmitochondria’Sfunctioncandirectlyinfluencethegrowthandproliferationofpreimplantationembryo．Ifmitochondriarespiratorychaingetimpaired，itwouldcauseATPreduced，aneuploidgenerated，andthedevelopm

5、entpotentialofovumsuppressedduetothedisturbanceofthenormalfertilizationandembryodevelopment．GRIM．19(GenesassociatedwithRetinoid—Interferon-inducedMortality．19)wasfoundtobeapartofcomplexIofmitochondrialrespiratorychainwhichcatalyzedthefirststepofelectrontransfe

6、rintheoxidativephosphorylationsystem(OXPHOS)．AnditsubsequentlywasdemonstratedtobeinvolvedincomplexI—IVassemblyandactivity，andmaintainingthemitochondrialtransmembranepotential(△Y1．GRIM一19。”blastocystsstrikinglydisplayabnormalmitochondrialstructure，morphology，an

7、dcellulardistribution．HomologousdeletionofGRIM-19causesembryoniclethalityatemoryomcday9．，．Subsequently．ithasbeendemonstrationthatGRIM-19wasessentialtoearlydevelopmentofheart．However，therewasnoreportabout7山东大学硕士学位论文GRIM一19inpreimplantationembryoofmice．So，inthis

8、researchwestudiedthefunctionofGRIM一19inpreimplantationembryoofmiceviadetectingtheexpressionofGRIM一19．MethodS1．SuperoVulationandeggscollectionSexualmaturityfemalemicewereabdominalinjectedwith10IUeachoneofhumanmenopausalgonadotropin(HMG)，and48hlaterinjectedwith1

9、OIUeachoneofhumanchorionicgonadatropin(hCG)．Onefemalemiceandonemalemiceasacouplewerebothplacedtogetherforfertilization．FertilizedovumwerecollectedwithG1mediumthenextday(27hafterhCG