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ID:32288281
大小:4.79 MB
页数:44页
时间:2019-02-02
《蛋白酶体抑制剂mg132对人结肠癌hct116细胞增殖和凋亡的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、蛋[J酶体抑制剂MG一132对人结肠癌HCT-116细胞增殖和凋产的影响中义摘嘤蛋白酶体抑制剂M6-132对人结肠癌HCT-116细胞增殖和凋亡的影响中文摘要目的:研究蛋白酶体抑制剂MG一132对体外培养的人结肠癌HCT一116细胞株增殖和凋亡的影响,并初步探讨其作用机制,为结肠癌的药物治疗提供依据。方法:采用不同浓度的(0.5一15umol/1)MG-132和阴性对照组作用于人结肠癌HCT-116细胞株,应用四甲基偶氮唑蓝(MTT)法检测吸光度值,计算对照组和各实验组细胞的生长抑制率;应用流式细胞仪(FCM)研究MG-132对
2、人结肠癌HCTll6细胞株细胞周期和凋亡的影响,计算细胞凋亡率;应用RT—PCR方法检测对照组和不同药物浓度组作用于人结肠癌HCT一116细胞后bcl一2、p27、caspase一3mRNA表达水平的变化;应用WesternBlot法检测细胞bcl一2、p27、caspase一3在蛋白水平的表达情况。结果:(1)MTT法显示不同药物浓度组均可抑制HCT-116细胞的生长,呈时间和剂量依赖性,实验组与对照组及各实验组之间差别均有统计学意义(P3、CT-116细胞均出现凋亡,可见细胞周期S期明显抑制,且随着浓度的增加HCT一116细胞周期GO/G1期比例增加,S期细胞比例下降,差异有统计学意义(P4、而增加,bcl-2蛋白表达随YlG一132浓度增加而下降(P5、殖和凋主堕影.堕EffectsofproteasomeinhibitorMG-132ontheproliferationandapoptosisofHumanColonCancerHCT-116cellAbstractObjective:ToinvestigatetheeffectofproteasomeinhibitoroncellproliferationandapoptosisofhumancoloncancercelllineHCT-116.alsotoexploreitspossiblemechanismsofanti6、-cancereffects.Methods:Invitro,humancoloncancercelllineHCT-116wastreatedbyproteasomeinhibitor(MG一132)indifferentconcentrations.bymeansofMTTassay,theabsorbancevaluewasdetectedandgrowthinhibitionratewascalculated.Cellcycleandapoptosiswereassessedbyflowcytometry(FCM):Th7、echangesofcellmorphologywereobservedbyinvertedandconfocalmicrocope.ThemRNAexpressionsofbcl-2、p27、caspase-3wereanalyzedbyreversetranscriplasepolymerasechainreaction(RT-PCR).WesternBlotwasusedtodetectbcl-2、p27、caspase-3proteinexpression.Results:(1)MTTassayindicatedthat8、theinhibitoryeffectofMG一132ontheproliferationofHCT..116cellWasfoundindose-and-timedependentmanner.Therewasgreatstatisticalsignifica
3、CT-116细胞均出现凋亡,可见细胞周期S期明显抑制,且随着浓度的增加HCT一116细胞周期GO/G1期比例增加,S期细胞比例下降,差异有统计学意义(P4、而增加,bcl-2蛋白表达随YlG一132浓度增加而下降(P5、殖和凋主堕影.堕EffectsofproteasomeinhibitorMG-132ontheproliferationandapoptosisofHumanColonCancerHCT-116cellAbstractObjective:ToinvestigatetheeffectofproteasomeinhibitoroncellproliferationandapoptosisofhumancoloncancercelllineHCT-116.alsotoexploreitspossiblemechanismsofanti6、-cancereffects.Methods:Invitro,humancoloncancercelllineHCT-116wastreatedbyproteasomeinhibitor(MG一132)indifferentconcentrations.bymeansofMTTassay,theabsorbancevaluewasdetectedandgrowthinhibitionratewascalculated.Cellcycleandapoptosiswereassessedbyflowcytometry(FCM):Th7、echangesofcellmorphologywereobservedbyinvertedandconfocalmicrocope.ThemRNAexpressionsofbcl-2、p27、caspase-3wereanalyzedbyreversetranscriplasepolymerasechainreaction(RT-PCR).WesternBlotwasusedtodetectbcl-2、p27、caspase-3proteinexpression.Results:(1)MTTassayindicatedthat8、theinhibitoryeffectofMG一132ontheproliferationofHCT..116cellWasfoundindose-and-timedependentmanner.Therewasgreatstatisticalsignifica
4、而增加,bcl-2蛋白表达随YlG一132浓度增加而下降(P5、殖和凋主堕影.堕EffectsofproteasomeinhibitorMG-132ontheproliferationandapoptosisofHumanColonCancerHCT-116cellAbstractObjective:ToinvestigatetheeffectofproteasomeinhibitoroncellproliferationandapoptosisofhumancoloncancercelllineHCT-116.alsotoexploreitspossiblemechanismsofanti6、-cancereffects.Methods:Invitro,humancoloncancercelllineHCT-116wastreatedbyproteasomeinhibitor(MG一132)indifferentconcentrations.bymeansofMTTassay,theabsorbancevaluewasdetectedandgrowthinhibitionratewascalculated.Cellcycleandapoptosiswereassessedbyflowcytometry(FCM):Th7、echangesofcellmorphologywereobservedbyinvertedandconfocalmicrocope.ThemRNAexpressionsofbcl-2、p27、caspase-3wereanalyzedbyreversetranscriplasepolymerasechainreaction(RT-PCR).WesternBlotwasusedtodetectbcl-2、p27、caspase-3proteinexpression.Results:(1)MTTassayindicatedthat8、theinhibitoryeffectofMG一132ontheproliferationofHCT..116cellWasfoundindose-and-timedependentmanner.Therewasgreatstatisticalsignifica
5、殖和凋主堕影.堕EffectsofproteasomeinhibitorMG-132ontheproliferationandapoptosisofHumanColonCancerHCT-116cellAbstractObjective:ToinvestigatetheeffectofproteasomeinhibitoroncellproliferationandapoptosisofhumancoloncancercelllineHCT-116.alsotoexploreitspossiblemechanismsofanti
6、-cancereffects.Methods:Invitro,humancoloncancercelllineHCT-116wastreatedbyproteasomeinhibitor(MG一132)indifferentconcentrations.bymeansofMTTassay,theabsorbancevaluewasdetectedandgrowthinhibitionratewascalculated.Cellcycleandapoptosiswereassessedbyflowcytometry(FCM):Th
7、echangesofcellmorphologywereobservedbyinvertedandconfocalmicrocope.ThemRNAexpressionsofbcl-2、p27、caspase-3wereanalyzedbyreversetranscriplasepolymerasechainreaction(RT-PCR).WesternBlotwasusedtodetectbcl-2、p27、caspase-3proteinexpression.Results:(1)MTTassayindicatedthat
8、theinhibitoryeffectofMG一132ontheproliferationofHCT..116cellWasfoundindose-and-timedependentmanner.Therewasgreatstatisticalsignifica
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