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1、中药复方中多糖的提取纯化及其对小鼠非特异免疫功能的影响AbstractChineseherbalmedicineistheChineseexcellenttraditionalculture'streasure,alongwiththesocialandscientificprogressanddevelopment,thehumancognitionandconstantlyupdatedhealthconcepts,Chineseherbalmedicineresearchathomeandabroad,alreadymoreandmoreexpected
2、attention.Therearepolysaccharide,flavonoids,nucleoside,organicacids,alkaloidetc.mainlybioactivesubstancesinChineseherbals.Withthedevelopmentofmolecularbiology,andgraduallyrealizethepolysaccharidesubstancesinanimals,plantsandmicrobesandsoonthebodycellsarefound,Constitutethelifeactivi
3、tiesofbiologicalmacromoleculesessentially(protein,nucleicacidandpolysaccharides),oneofthebasicmaterial,participateincellsofthelifeactivitiesofadjustment.TheresearchersfoundthatChineseherbalmedicineofpolysaccharidescomponentshaveregulateimmune,antitumor,antiviral,delayageing,antioxid
4、ation,fallhematicfat,fallbloodsugar,anti-infectionetc.variousphysiologicalactivityfunction,oneoftheimmunityactivitymuchresearchersattention.Thisexperimentusinganhydrousalcoholprocessingherbalpowder,waterextractiontotalpolysaccharidescomposition,andstudyontheseparationandpurification
5、technologyofPolysaccharide,andresearchedtheeffectsonimmunityfunctioninmice.1、TheChineseHerbalMedicineCompoundofcrushingdegreasingasrawmaterials.Accordingtothefollowingextractionconditionsforextraction:extractingtime24hours,theratioofmaterialtosolvent1:10(ChineseHerbalMedicineCompoun
6、d:Water)andtimesofextracting3.AfterextractioncalculationratioTheChineseHerbalMedicineCompoundtotalpolysaccharidecanreach43.85%.Aftertheethanolclassification,A1,A2,A3polysacchariderespectivelythepurityof49.6%,50.5%,54.7%.2、ToAB-8,LSA-5B,DM-18,CAD-40,LX-1,D941sixkindsofmacroporousadso
7、rptionresinformaterial,throughthestaticanddynamicadsorptionanddesorptionexperiment,westudiedtheabove6kindsofresinadsorptionanddesorptionperformanceofcompoundpolysaccharide.Resultsshowthat:AB–8resinispreferablecandidateforseparatingcompoundpolysaccharide.3、Thoughsingle-factorandortho
8、gonalexperimentdesi