消癌平注射液与其与奥沙利铂联合抑制肝癌细胞增殖与诱导凋亡的的研究

《消癌平注射液与其与奥沙利铂联合抑制肝癌细胞增殖与诱导凋亡的的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、摘要关键词：消癌平；奥沙利铂；原发性肝癌；HepG一2细胞；细胞增殖；细胞凋亡南京中医药大学硕?I：学位论文AbstractObjective：ObservetheeffectsofMarsdeniatenacissimainjectionaloneandtheeffectswhenitiscombinedwithoxaliplatinonhumanhepatomacelllinesHcpG-2invitro，andinvestigatethemechanism，toprovidethetheoreticalfoundationforthecombin

2、ationofchemotherapywithtraditionalmedicinetocurehepatocarcinoma．Methods：UsevariousmethodstodetectthegrowthconditionofhumanhepatomacelllinesHepG-2invi仃owhentheyaleaffectedbydifferentconcentrationofMarsdeniatenacissimainjection，oxaliplatinaloneandbythecombinativeuseofthedrugsabove

3、：(1)EstimatingtheinhibitionofproliferationbyMTT-test；(2)Observingthemorphologicchangesbylightmicroscope；(3)ObservingthedyednucleolusofapoptosiscellbyTUNELmethod；(4)Observingthemorphologiccharacteristicsofapoptosisbyfluorescencemicroscope；(5)Analyzingthedistributionofcellcycleand

4、earlyapoptosisbyflowcytometry；(6)Detectingtheexpressionsofapoptosis-associatedgeneproteins：Survivin，Bcl一2andCaspase-3byimmunohistochemicaltechnique．Results：(1)MarsdeniatenacissimainjectioncouldinhibittheproliferationofHepG-2celllines，andtheinhibitiondependsontheexposuretimeanddo

5、se；thereworeadditiveeffectswhencombineMarsdeniatenacissimainjectionof17．51d／mlwithoxaliplatinof0．5I-tg／ml；(2)Observedbylightmicroscope，theCellsshrankandturnedround，andthecontactsamongcellsbecameloose；(3)TheTUNELmethoddetectedthatthenucleusesofapoptoticcellsweredyeddeepbrown，thea

6、poptosisindex(ADincreasedwhencombinedwithoxaliplatin,andthedifferenceissignificantstatistically；(4)Observedbyfluorescencemicroscope，theapoptosistookplaceincells，thephenomenonbecomemoreobviouswhenMarsdeniatenacissimainjectioncombinedoxaliplatin(P

7、howedthatMarsdeniatenacissimainjectionblockedcellsatGo／GtphasewhilethecombinationofMarsdeniatenacissimainjectionandoxaliplatinblockedcellsatSphase；TheanalysisofcellcycleshowedthatMarsdeniatenacissimainjectioncanmakecells’apoptosistakeplace，thephenomenonbecomemoreobviouswhenMalsd

8、eniatenacissimainjectioncombinedoxaliplatin(P