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ID:32256395
大小:3.74 MB
页数:55页
时间:2019-02-02
《炎症免疫和良性前列腺增生进展的相关性的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、天津医科大学硕七学位论文中文摘要背景与目的:良性前列腺增生是引起老年男性下尿路症状的常见原因。在5l~60岁的男性中组织性良性前列腺增生发生率是41%,61~70岁占70%,81~90岁达90%。伴随我国社会结构老龄化的加重,前列腺增生症在我国的发病率也同趋上升,但尚没有一种理论能够确定良性前列腺增生发生发展的决定性因素。近年来组织学前列腺炎的存在被认为是影响良性前列腺增生临床进展的一项重要危险因素。我们采用单克隆抗体免疫组化技术检测CD8、CD20、IL.6、IL.23和TGF.B在增生前列腺组织中的表达,将不同检测结果患者的临床数据进行对比分析,探讨炎症免疫在良性
2、hlfYIJ腺增生发生与进展中的作用,为良性前列腺增生病因学的进一步明确提供理论支持。材料与方法:本研究选取2009年7月至2010年9月间,60例在天津医科大学第二医院泌尿外科行经尿道前列腺切除术或经膀胱fj{f列腺摘除术患者的临床资料及手术标本蜡块。以上患者年龄46----85岁,中位年龄70岁(P25=62岁,P75=76岁)。术后经我院病理科HE染色证实均为单纯良性前列腺增生。采用亲和素一生物素一过氧化物酶复合物免疫组化法(StreptActividin.BiotinComplex,SABC法)进行检测。染色步骤按SABC免疫组化法进行操作,结果判定以光镜下观
3、察细胞出现黄色颗粒为阳性染色,棕黄色颗粒为强阳性染色。应用SPSS17.0统计软件包对所有数据进行统计学分析,P<0.01为差异有统计学意义。结果:前列腺增生组织中的CD8的阳性表达率为83%(50/60),CD20的阳性表达率为60%(36/60),IL.6的阳性表达率为78%(47/60),IL一23的阳性表达率为55%(33/60),TGF.D的阳性表达率为63%(38/60)。在增生腺细胞内CD8、CD20、IL.6、IL.23表达呈多数阳性的病例组中,前列腺体积较大(P<0.01),I-PSS评分更高(P4、阴性病例组相比,术前最大尿流率更低,组间差异具有统计学意义(尸5、orderintroducinglowurinarytractsymptomesoftheagedmalepopulation.Anestimated42%ofmen51to60yearsofagehavehistologicalBPH.Theincidenceincreasestoover70%inmen61to70yearsofageandtoalmost90%inthose81to90yearsofage.Wimrelatedofsocialstructureaging.theincidenceofBPHinoBrcountrybecomehigher.Butb6、utnotheoryshowthedependentroleinthepathogenesisofBPH.InthelastyearshistologyinflammationappearstobepotentiallyimportantintheprogressionofBPH.Inthisresearchment,immunohistochemicalanalysisWasusedtoexaminetheexpressionofCD8,CD20,Interleukin一6,Interleukin一23andTGF—pinBPHtissues.Mainclinica7、ldatawerecomparedandanalyzedbetweengroupsofvariantexpressionresults.Weintendedtoinvestigatetheeffectimmune-inflammationinthedevelopmentandprogressionofBPH..MaterialsandMethod:Atotallyof60pmientsofBPHfromdepartmentofurology,secondhospitalofTianjinmedicaluniversityundergoingsurgi
4、阴性病例组相比,术前最大尿流率更低,组间差异具有统计学意义(尸5、orderintroducinglowurinarytractsymptomesoftheagedmalepopulation.Anestimated42%ofmen51to60yearsofagehavehistologicalBPH.Theincidenceincreasestoover70%inmen61to70yearsofageandtoalmost90%inthose81to90yearsofage.Wimrelatedofsocialstructureaging.theincidenceofBPHinoBrcountrybecomehigher.Butb6、utnotheoryshowthedependentroleinthepathogenesisofBPH.InthelastyearshistologyinflammationappearstobepotentiallyimportantintheprogressionofBPH.Inthisresearchment,immunohistochemicalanalysisWasusedtoexaminetheexpressionofCD8,CD20,Interleukin一6,Interleukin一23andTGF—pinBPHtissues.Mainclinica7、ldatawerecomparedandanalyzedbetweengroupsofvariantexpressionresults.Weintendedtoinvestigatetheeffectimmune-inflammationinthedevelopmentandprogressionofBPH..MaterialsandMethod:Atotallyof60pmientsofBPHfromdepartmentofurology,secondhospitalofTianjinmedicaluniversityundergoingsurgi
5、orderintroducinglowurinarytractsymptomesoftheagedmalepopulation.Anestimated42%ofmen51to60yearsofagehavehistologicalBPH.Theincidenceincreasestoover70%inmen61to70yearsofageandtoalmost90%inthose81to90yearsofage.Wimrelatedofsocialstructureaging.theincidenceofBPHinoBrcountrybecomehigher.Butb
6、utnotheoryshowthedependentroleinthepathogenesisofBPH.InthelastyearshistologyinflammationappearstobepotentiallyimportantintheprogressionofBPH.Inthisresearchment,immunohistochemicalanalysisWasusedtoexaminetheexpressionofCD8,CD20,Interleukin一6,Interleukin一23andTGF—pinBPHtissues.Mainclinica
7、ldatawerecomparedandanalyzedbetweengroupsofvariantexpressionresults.Weintendedtoinvestigatetheeffectimmune-inflammationinthedevelopmentandprogressionofBPH..MaterialsandMethod:Atotallyof60pmientsofBPHfromdepartmentofurology,secondhospitalofTianjinmedicaluniversityundergoingsurgi
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