麦冬多糖和皂苷的分离和活性的的研究

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1、西南科技大学硕士研究生学位论文第1I页和02‘．的能力有显著的增强。麦冬皂苷也表现出极强的自由基清除活性，其清除能力的变化要高于多糖。5、对多糖和皂苷进行体外免疫活性研究。结果表明各多糖组分和皂苷均表现出增强巨噬细胞吞噬能力、增强巨噬细胞能量代谢和促进巨噬细胞分泌NO和IL．1的活性。NP添加组作用效果不明显。AP1在低浓度时表现出较低的促进作用，添加浓度为400gg／mL时呈现较高的促进作用。AP2、AP3、CP、S-NP和皂苷均表现出较高的活性，呈剂量依赖关系，其活性强弱顺序为S．NP>AP3>皂苷>C

2、P>AP2。关键词：麦冬皂苷多糖分离纯化自由基清除活性免疫活性西南科技大学硕士研究生学位论文第1II页AbstractOphiopogonjaponicusKer—Gawler(Liliaeeae)isoneofthemostfrequentlyusedChineseherbs．ThemainchemicalcompositionsofO．japonicusaresaponins，polysaccharides，homoisoflavonoids，andSOon．O．japonicushasdrawnbro

3、adattentionforitsvariouspharmacologicalandphysiologicalfunctions，suchasanti-myocardialischemia，anti-thrombosis，enduringhypoxia，decliningblood-sugarandSOon．Butthereportonitscomprehensiveutilizatitionwasrelativelyless．Inthisworkwestudiedtheextractionproceess

4、，purificationtechnology，radicalscavengingandimmunoregulatoryactivityofpolysaccharidesandsaponinsfromO．japonicus，whichwereplantedinSantaicounty，Sichuanprovince．Theprimaryresultsareasfollows：1．Theoptimalconditionfortheextractionoftotalpolysaccharideandsaponi

5、nwerestudied．Thebestconditionsofextractingpolysaccharideweretemperature，70℃：solid／liquid，1：1O；refluxtime，1h；extraction，2times．Thebestconditionsofextractingsaponinweretemperature，60"C：ethanol，70％；solid／liquid，1：15；refluxtime，1．5h；extraction，2times．2．Fourpol

6、ysaccharidefractions(NP,AP1，AP2andAP3)wereisolatedandpurifiedbyDEAE-SepharoseFFandSepharose6一FFcolumnchromatography．NPwassulfatedtoobtainS．NP．ThecontentsofhexuronicacidandsulfatedecreasedintheorderAP3>AP2>AP1>NP(S—NP)andNP．M>AP3>AP2>AP1>NPrespectively．GCan

7、alysisresultsshowedthatNPwascomposedofmannoseandglucose，AP1wascomposedofmannose，rhamnose，xylose，arabinoseandglucose，AP2wascomposedofrhamnoseandglucose，AP3wascomposedofrhamnoseandglucose．3．PurificationtechnologyoftotalsaponinbyHZ-801，HZ一806，D101，X一5，AB．8，D0

8、01，DA201，D315macroporousresinswerestudied．D101macroporousresinsexhibitedthebestcharacteristicsandthenfurtherexplored．Thebestconditionwere：adsorptiontime：50min，resinquantity：medicinalherbsontheamountofsample1：