α肾上腺素能受体激动对tlr4信号通路影响的研究

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1、白组比较无明显升高(p>O．05)；2、TLR4mRNA干扰后Q受体激动剂NE刺激血液离心后上清液中IL一6及IL．18、单核细胞NF．1cBP65蛋白的表达为：Si+NE75组均明显低于空白组、So+NE75组及NE75组(P<0．05)；3、分别阻断0【受体、P38MAPK、PKA、PKC后，用有效浓度NE(75阻nol／L)刺激外周血单核细胞，观察TLR4蛋白的表达量为：Gt．blocker组、P38组、PKA组与NE75组比较均明显降低‘(p0．05)，PKC组与NE75组及空白组比较均明显增加(p<0．05)。结论

2、：1)Q受体激动剂呈剂量依赖性诱导人外周血单核细胞TLR4信号通路激活，以759mol／L去甲肾上腺素为最佳剂量；2)a受体、P38MAPK、PKA、NF．r,BP65介导了o【受体激动剂诱导的TLR4信号通路激活。关键词：TLR4Q．肾上腺素能受体激动剂信号通路ThecrosstalkbetweenQ-adrenoceptoragonistinducedandToll--likereceptor4mediatedsignalingpathwayMasterdegreeCandidate：ZhangXingSupervisor：Prof．QuPengMajor：Inter

3、nalMedicineAbstractObjective：ToinvestigationoftheeffectonT0ll—likereceptor-4(TLR4)mediatedsignaltransductionandtheassociatedfactorinperipherybloodmonocytesduetotheactivationofa··adrenoceptor,toexploretheinteractionofa--adrenoceptorandTLR4andtheregulatorymechanisminvolved．Methods：Bloodsamp

4、leswerecollectedfromhealthyyoungpeopleatperipheryandfreeze-conservedfor／nvitroexperiments．(1)T0ex抽ainetheeffectofa-adrenoceptoragonistnorepinephrineOllproteinandmRNAofTLR4，P38mRNA，NF—r,Bp65inmonocytesofperipherybloodandinflammationfactorinterleukin-6(IL-6)andinterleukin-18(IL一18)inserunl．

5、Theblooddonorswere10healthyyoungpeopleandcollected32mlperipheryvenousbloodindividual．Aftertreatment、丽tllanticoagulantEDTA，Thebloodsamplesweregroupedtoblankgroup，25lamoULNEgroup，50lamol／LNEgroupand75lamol／LNEgroup．AndafterincubationwithdifferentdoseofNEabove，ELISAmethodandflowc”ometryWasus

6、edtodeterminetheconcentrationofIL—·6andIL--18inserumandthepercentageofTLR4positiveCD14+monocytes，irrespectively．DeterminationtheexpressionofTLR4mRNA，P38mRNAuseRT-PCRandtheexpressionofNF-r,Bp65useimmunocyto··chemistrymethod．(2)AfterbeingTLR4mI斟AsilencingmediatedbysiRNAmethod，observationthe

7、concentrationinfluenceofassociatedfactorsstimulatedby仅-adrenoceptoragonistnorepinephrine．111eblooddonorswere10healthyyoungpeopleandcollected16mlperipheryvenousbloodindividual．AftertreatmentwithanticoagulantEDTA，111ebloodsamplesweregroupedtoblankgroup，75pmol／LNEgroup