不同异种抗原胸腺修饰对延迟性异种排斥反应的作用的研究

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1、不间异种抗原胸腺修饰对延迟性异种排斥反应的作用研究中文摘要升均显著减缓(P0．05)。④移植后受体外周血VCAM一1和ICAM．1的变化：移植后C，D两组VCAM—l和ICAM-1水平上升均显著减缓(P<0．05)，D组与c组比较有显著差异(P<0．05)。⑤病理：供心停跳时四组排斥心脏病理表现均呈典型DXR表现。结论：①异种抗原胸腺修饰可抑制小鼠到大鼠心脏移植中受体T细胞的增殖、抑制受体诱生抗体IgG的产生，延长移植物存活时间，但不能完全克服DXR的发生。②供体血管内皮细胞胸腺修饰可减轻移植物

2、血管内皮细胞的激活，其诱导效果比脾细胞胸腺修饰好。③ICAM一1和VCAM一1的表达水平与延迟性异种排斥反应的发生和发展有关，可作为异种器官移植治疗干预的靶分子之一，为异种移植免疫排斥治疗开拓新的方向。关键词：胸腺修饰异种心脏移植延迟性排斥反应血管内皮细胞Il研究生：夏漫辉导师：沈振亚教授不同异种抗原胸腺修饰对延迟性异种排斥反应的作用研究英文摘要TheEffectofIntrathymicInoculationtODelayedXenograftRejectionwithDifferentXenogeneicAntigensABSTRAC

3、TObjectiveThisstudywasdesignedtoinvestigatetheeffectofintrathymicinoculationwithxenogeneicantigensandwholebodyirradiationonthedelayedxenograftrejection(DXR)inthemodelofmousetoratheartxenotransplantation，toinquiretheeffectofTlymphocytesindelayedxenografirejectionandtheimpr

4、essionoffunctionofvascularendothelialsystemwithintrathymicinoculation．MaterialsandmethodsDonor(masculineNIHmouse)andrecipient(masculineSDrat)weredividedrandomlyintofourgroups．GroupA(Controlgroup)：withoutpretreatment；GroupB(WBIgroup)：therecipientsreceived3．5GyC0607-raywhol

5、ebodyirradiation(WBI)17daysbeforetransplantation；GroupC(WBIplusintrathymicinoculationwithmouseSCsgroup)：afterWBI，therecipientthymuswasinjectedwiththemouseSCs(spleencells)5x1063dayslater，andGroupD(WBIplusintrathymicinoculationwiththemouseVECsgroup)：afterWBI．therecipientthy

6、muswasinjectedwiththemouseVECs(vascularendothelialcells)5x1063dayslater．Afterdifferentpretreatmentstotherecipients，theabdominalheterotopichearttransplantationfrommousetoratwereperformed．Thegraftedheartmeansurvivaltime(MST)，theresultsofMCR(mixedcellreaction)beforetransplan

7、tationwereobserved．ThechangeofantibodyIgGoftherecipientbeforeandaftertransplantationwasdetectedbytheflowcytometry(FCM)．ThechangeofVCAM一1andICAM一1oftherecipientaftertransplantationwasdetectedbyELISA：Results(!)MST：GroupD(156．2士：lq．7h)>GroupC(125．6"18．2h)>GroupB(94．44-21．5h)

8、>GroupA(59．94-4．7h)．GroupDandGroupCwereprolongedsignificantly(P<0．05)，andGroupDwaslongerthanGrou