低硒对发育期大鼠脑中creb与核基质蛋白影响的研究

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1、性细胞数表示p-CREB在大脑皮层（对照组＝171.89±63.062，低硒组＝109.44±38.41，P<0.01）海马（对照组＝28.14±9.25，低硒组＝8.67±1.53P<0.05）的改变，显示低硒组较对照组均明显降低，且p-CREB在星形细胞中（对照组＝157.17±44.80，低硒组＝29.40±13.11，P<0.01）较胶质细胞（对照组＝68.80±13.78，低硒组＝51.50±14.86，P>0.05）下降明显（分别下降81.3％，25.1％）。利用高效毛细管电泳CZE分离模式能够将NMPs分离出3~4个蛋白峰，波形较稳定，进样9m

2、in左右有一个蛋白峰低硒组明显低于对照组。结论（1）低硒膳食能够引起发育期大鼠大脑皮层和海马的氧化代谢水平降低。（2）CREB表达减少反映低硒能够引起大脑皮层和海马神经元功能下降，提示低硒可能导致学习记忆能力降低的潜在因素之一。（3）低硒能够引起脑NMPs的变化。关键词硒脑cAMP反应元件结合蛋白核基质蛋白高效毛细管电泳万方数据TheEffectsofSeleniumDeficiencyDietonCREBandNuclearMatrixProteinsinBrainofDevelopingRatPostgraduate:GengYiqunSuperviso

3、r:Prof.XuXiaohuProf.SuMinAbstractObjectToobservethechangesoflipidperoxideinthecortexandhippocampusofdevelopingratinducedbyseleniumdeficiencydietandstudytheneuronfunctionbythecontentandactivitydetectionofcAMPResponseElement-BindingProtein(CREB)incortexandhippocampus.Tostudythealtera

4、tionofNMPscomponents.MaterialandmethodGroupsofhealthyadultSDratswithequalmaleandfemaleatrandomwerefedwithseleniumdeficiencyandseleniumadequatefood.Thethirdgenerationat21daysofagewerekilledandthecortexandhippocampusweredissectedonicerapidly.ThecontentsofMDAwereassayedby2-thiobarbitu

5、ricacid(TBA)test.TheexpressionofCREBandp-CREBwereexaminedbywesternblotintheextractedtotalproteinfromcortexandhippocampus.TheexpressionandthelocationofCREBandp-CREBwerefurtherexploredindifferentkindofneuroninthesameregionofbrainbyparaffinbrainsectionimmunohistochemistry.Theexpressio

6、nofp-CREBwasalsodetectedfromtheextractedNMPsofratbrainbywesternblot.ThecomponentoftheNMPsbetweentwogroupswereanalysedandcomparedbyHighPerformanceCapillaryElectrophorisis(HPCE).ResultThecontentofMDAinthecortexandhippocampusofseleniumdeficiencygroupwasmuchhigherthanthatofthecontrolgr

7、oup(controlcortexMDA＝0.31±0.13,seleniumdeficiencycortexMDA=0.66±0.10,P<0.05;controlhippocampusMDA=0.42±0.11，seleniumdeficiencyhippocampusMDA=0.89±0.066);The万方数据detectionofCREBandp-CREBfromthetotalproteinshowedthattheexpressionsofseleniumdeficiencygroupwerelowerthanthatofcontrolgrou

8、p.ThegreydegreeofCREBposit