小分子蛋白sulfiredoxin-1对大鼠星形胶质细胞氧化应激损伤后细胞凋亡的保护作用与 机制分析

《小分子蛋白sulfiredoxin-1对大鼠星形胶质细胞氧化应激损伤后细胞凋亡的保护作用与 机制分析》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、万方数据重庆医科大学硕士研宄生学位论文SULFIREDoXIN．1AMELIoR√气TESoXIDArrIVESTRESSIN叫RYINDUCEDBYoXYGEN．GLUCoSEDEPRIV盯IoNANDHYDRoGENPEROXIDEINRArCoRTICALASTROCYTESVIAANTI．APoPTOSISBackground：ABSTRACTAstrocytesapoptosisplaysavitalroleinCNSinjuries，suchascerebralischemia，Alzheimer’Sdiseas

2、e，andParkinson’Sdisease．Sulfiredoxin．1(Srxn1)，acentralendogenousantioxidantprotein，hasimportantneuroprotectivefunctionsincerebralischemiaincludingantioxidationandanti．apoptosis．However,themechanismsofSrxnlduringapoptosisincerebralischemiahavenotbeenwellstudied．Obje

3、ctive：TodetermineheroleofSrxnlinprotectionfromapoptosisinducedbyOGDorH202anditspossiblemechanism．Methods：Weusedlentiviralvector-encodingshorthairpinRNAs(shRNAs)totransfecttoastrocyteswithinterferenceSrxnl，andthenexposedthemtoOGDfor6hor100gMH202for6htOmodeloxidative

4、stressinjury．(2)WemeasuredcellviabilityandcelldamageasdeterminedbyMTSandLDHassays．万方数据重庆医科大学硕士研究生学位论文(3)UsingimmunocytochemistryHoechst33342andflowcytometryAV／PIdoubledyetodetectcellapoptosis．(4)JC一1probeandFluo一3，AMprobewereusedtodetectthechangeofmitochondrialmemb

5、ranepotentialandintracellularfreeGa2+usingLaserconfocal(5)UsingWBtomeasuretheproteinofSrxnl，cyt．C，caspase一9，caspase-3，PARRbax，bcl-2，andcaspase一12；andQPCRtomeasurethemRNAlevelofSrxnl．Results：(1)TheresultsofMTSandLDHshowedthatinterferenceSrxnlattenuatedcellviabilitya

6、ndincreasedcelldamage(p<0．05，p<0．01)．(2)Hoechst33342andFCMshowedthatinterferenceSrxnlincreasedapoptosis(p<0．O1)．(3)JC-1stainingshowedthatinterferenceSrxnlinduceddissipationof△、

7、，mp

8、3，caspase-9，PARRbax，anddeclinedantiapoptoticbcl-2proteinasdeterminedbyWB(p<0。05，p<0．01)．Buttherewasnoobviouschangeincaspase-12proteinafterinterferenceSrxnlp>O．05)(5)Fluo一3，AMstainingshowedthatafterinterferenceSrxnlthelevelofintracellularCa。werenotdifferentfromtheco

9、ntrolgroup(p>0．05)6万方数据重庆医科大学硕士研究生学位论文Conclusion：TheseresultssuggestedthatSrxnlcanprotectprimaryratcorticalastrocytesfromOGD·-orH202·-inducedcell