腺苷预处理对糖氧剥夺损伤星形胶质细胞的保护作用

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1、腺昔预处理对糖氧剥夺损伤星形胶质细胞的保护作用摘要：目的：观察糖氧剥夺对星形胶质细胞的影响及腺昔的保护作用。方法：取体外培养第三代或第四代星形胶质细胞，传代至96孔板或24孔板内，细胞贴壁并长出突起后，随机分为三组：正常对照组(normalcontrolgro叩)、糖氧剥夺组(OGDgroup)s腺昔预处理组(ADOgroup),进行糖氧剥夺/复氧处理。观察糖氧剥夺8h复氧糖24h后星形胶质细胞形态、细胞活性。结果：糖氧剥夺复氧糖24h后，正常对照组星形胶质细胞生长状态良好;OGD模型组星形胶质细胞胞体水肿变圆，细胞形

2、态由不规则变成梭形，突起明显变短或消失；ADO组细胞水肿较轻。OGD模型组星形胶质细胞活力下降，OD值明显低于正常对照组(P<0.01)；ADO组细胞活力显著高于OGD模型组，表明ADO能明显改善细胞活力(P<0.01)o结论：糖氧剥夺可引起星形胶质细胞严重损伤,星形胶质细胞形态、活性与缺氧缺糖呈对应变化，腺昔能减少糖氧剥夺后星形胶质细胞形态学改变及细胞凋亡，提示腺昔对糖氧剥夺的星形胶质细胞有一定的保护作用。关键词：腺昔；星形胶质细胞；糖氧剥夺TheprotectionofAdenosinePreconditionin

3、gonOxygen-glucosedeprivationinjuryinducedastrocytecells[Abstract]ObjectiveToinvestigatetheeffectofoxygen-glucosedeprivationandprotectiveeffectofadenosineonastrocytes.MethodsAstrocyteswereculturedforthirdorfourthgenerationinvitro,andthenpassagedat96or24wellplate.

4、Whentheastrocytesattachingtothewallandgrowingwithprominences,theywererandomlydividedinto3groups,normalcontrolgroup,oxygen-glucosedeprivation(OGD)groupandadenosinepreconditioning(ADO)group.ThecellsweretakenOGD/reoxygenationtreatment.Themorphologyandactivityofastr

5、ocytecellswereobservedat8hoursafterOGDand24hoursafterreoxygenation.ResultsAt24hoursafterOGD/reoxygenationtreatment,theastrocytecellswerewithnormalmorphologyinnormalcontrolgroup；thesomaofastrocytecellsbecameedemaandroundinOGDgroup,thecellmorphologychangedtofusifo

6、rmisfromirregular,andprominenceobviouslybecameshortordisappeared；theedemawaslighterinADOgroup・ActivityofastrocytecellsdecreasedinOGDgroup,andODvaluewassignificantlylowerthanthatinnormalcontrolgroup(P<0.01).CellactivitywashigherinADOgroupthanthatinOGDgroup,whichi

7、ndicatedthatADOcanobviouslyimprovedcellactivity(P<0.01)・ConclusionsAstrocytesareinjuredseriouslyafterOGD；morphologyandcellactivityofastrocytestakechangeinaccordancewithoxygendeficitandglucosedeprivation.Adenosinecandecreasemorphologicalchangesandcellapoptosisofa

8、strocytecellsafterOGD.wecanmakeconclusionthatadenosinehasinfluenceonastrocytesafterOGD.【Keywords]Adenosine；Astrocyte；Oxygen-glucosedeprivation【中图分类号】Q2-09【文献标识码】B【文章编