维药神香草总黄酮对哮喘大鼠气道炎症和气道重塑的影响与其作用机制分析-(11558)

《维药神香草总黄酮对哮喘大鼠气道炎症和气道重塑的影响与其作用机制分析-(11558)》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、新疆医科大学硕士学位论文TheeffectandmechanismofTraditionalUyghurMedicinalherbHyssopusofficinalisL．TotalFlavonoidsonairwayinflaromationandremodeHng。ratsmodelnalrwaYinIlammatlonanremoaennginaratsmoelJ●●■J1oICnronlCaStllmaPostgraduate：Ayxam．TaxSupervisor：Prof．Abdiryim．YusupAbstracto

2、bjective：TheprobtoHyssopustotalflavonoidsfromcellularandmolecularlevelofasthmaairwayinflammationandairwayremodelingmechanismMethods：(1)Thetotalflavonoidsadsorptionanddesorptioncapacityasindextocompare1differenttypesofmacroporousresinsandScreeningofthebestmacroporousre

3、sin，Staticadsorption—elutiontestwerecarriedout．SinglefactortestWasusedtoinvestigatethebestpurificationtechnologyofmacroporousresin．(2)Throughtheadoptionofovalbumin(OVA)injectionatomizationinhalationmethodasthmamodelinrats，observetheeffectofantiasthmaofHyssopustotalfla

4、vonoidsonguineapigswerestudied．BronchialasthmaticmodelWasestablishedbyovialbuminsensitizationandlong—terminhalationprovocation．ThentheratsofeachtreatmentgroupwereintervenewitllthedexamethasoneofHyssopustotalflavonoids．UsingtheFlowtypeintegralplethysmography(whole-body

5、plethysmography,WBP)systemdetectedtheeffectsonlungfunction；theratslungtissuebeingdyedwithHE，Massonweremadepathologicalsection．UsingELISAkitsmethoddetectedthecontentofIgElevel、忻thasthmarats’serumanddetectedthecontentofeotaxinlevel．TGF一131level．CollIIlevelwitllasthmarat

6、slungtissue．Results：(1)AB-8macroporousresinwasthemostsuitableoneforpurificationoftotalflavonoidsinHyssopusofficinalisL．Theoptimumpurificationparameterswereasfollowings：thesampleconcentration2．56mg’mL-1，sampleflowrate4BV+h-1，Resinbeddiameterhighratioof1：5，Theresinwaswa

7、shedwith5BVwater,theelutionflowrateof5BV／handtheeluentwasdiscardedandelutedwith9BV70％ethanolat4BV．h-1；TheEluateWaSconcentratedanddriedto3新疆医科大学硕士学位论文obtainthetotalflavonoidsformHyssopusofficinalisL．．Thispurificationconditionstomakethetotalflavonoidsconcentrationreache

8、d65．6％．(2)Therespiratoryrate(F)inpulmonaryfunctiontestinthemodelgroupincreasedsignificantlycomparedwithnormalgroup(P<0．oi)，M