农杆菌与花粉管通道法介导‘魏可’葡萄遗传转化体系的建立

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1、农杆菌及花粉管通道法介导‘魏可’葡萄遗传转化体系的建立3．Nineplantsof‘Wink’grapewithKarlresistancewereobtainedbyoptimizingAgrobacteriummediatedtransformation．TherewerefourPCRpositiveplantsandoneRT-PCRpositiveplantdetectedbyPCRandRT-PCR．ThesequenceshowedthattheamplifiedfragmentsofnptEgenehadb

2、eenintegratedintothehostgenomeof‘Wink’grape．Thetestresultsofkanamycin-resistantexperimentshowedthattheabilityofKanresistanceWaSmuchbettercamparedthePCRpositiveplantswimthecontr01．Itprovedthatintroducednpt／／genecouldexpressin‘Wink’grape．4．Pollen．tubemediatedtransfo

3、rmationWaSadoptedtointroducethenptffgeneintotheinflorescenceof‘Wink’grapevine．Onehundredandf0啊plantletswereobtained．AfterscreeningseventyplantletsbythewayofPCRandRT-PCR,eightplantsshowedPCRpositive，andtwoshootsshowedRT-PCRpositive，whichinitiallydemonstratedthatthe

4、npt／／geneWaSintegratedintothegenomeof‘Wink’grapevine．AfterinoculatingthePCRpositiveplantsonMS+Kan20mg／L．Itwasobservedthatthecolouroftheleaveshadbeenchanged．Sevendayslater,partoftheCKleavesbecameyellow,andbrowningspotsappeared．Howerver,theleavesofPCRpositiveplantsr

5、emainedgreen．Itshowedthatnpt／／genewhichwereintegratedin‘Wink’grapecouldexpress．KEYWORDS：‘Wink’grapevine；Regenerationsystem；Agrobacterium-mediated；nptEgene；Pollen-tubepathwayIV缩略词缩略词Abbreviation英文缩写英文名称中文名称AS6．BACbCDNACefCTf蛆DEPCDNAEBEDTAGUSIBAKanLuxNPTIIPCRPVP砌fRN

6、ARr-PCRSDSTDZAcetosyringone6-benzylaminopyrineCarbenicinllinComplementaryCefotaximeCetyltrimethylammoniumbromideDiethylpyrocarbonateDeoxyribonucleicacidEmidiumBromideEthyleneDiamineTetraaceticAcia13-glucuronidaseIndolebutyricacidKanamycinLuxembourgNeomyeinePhospho

7、transferaseIIPolymerasechainreactionPolyvinylpyrrolidoneRifampicinRibonucleicacidReversetranscriptionPolymerasechainreactionSodiumdodecylsulfate1-Phenyl-3-)1，2，3-Thiazol·5-YL)Urea乙酰丁香酮6．苄氨基腺嘌呤羧苄西林钠互补DNA头孢霉素十六烷基三甲基溴化胺焦碳酸二乙酯脱氧核糖核酸溴化乙锭乙二胺四乙酸p-葡萄糖苷酸酶吲哚丁酸卡那霉素米．烛光新霉素磷酸转

8、移酶聚合酶链式反应聚乙烯吡咯烷酮利福平核糖核酸反转录聚合酶链式反应十二烷基磺酸钠噻重氮苯基腺TrisTrishydroxymethylaminomethane三羟甲基氨基甲烷X-Glucg淼lucu瑚ron?lde№。抽面舛廿。薯蒸秽p。匍萄耩醑酉旨V第一章文献综述葡萄(VitisL。)属落叶藤本植物，